Colby S R
Science. 1965 Oct 29;150(3696):619-20. doi: 10.1126/science.150.3696.619.
The N-glycoside of the herbicide 3-amino-2, 5-dichlorobenzoic acid (amiben) was synthesized in the laboratory. The compound was chromatographically identical with a conjugate of amiben isolated from soybean plants, Glycine Max (L.) Merr. The seeds of a tolerant plant, soybean, and of a susceptible one, barley (Hordeum vulgare L.), were incubated in amiben labeled with carbon-14, and the seedlings were extracted. Essentially all the radioactivity in soybean was present as a compound corresponding to N-glycosyl amiben. Only a small amount of this conjugate was present in barley; most of the radioactivity was present as free amiben or as other unidentified metabolites.
在实验室中合成了除草剂3-氨基-2,5-二氯苯甲酸（敌草灭）的N-糖苷。该化合物在色谱上与从大豆植株（大豆，Glycine Max (L.) Merr.）中分离出的敌草灭共轭物相同。将耐受植物大豆和敏感植物大麦（Hordeum vulgare L.）的种子在标记有碳-14的敌草灭中培养，然后提取幼苗。大豆中基本上所有的放射性都以与N-糖基敌草灭相对应的化合物形式存在。大麦中仅存在少量这种共轭物；大部分放射性以游离敌草灭或其他未鉴定的代谢物形式存在。
