人-鼠体细胞杂种中HL-A共同抗原决定簇的染色体定位

Chromosomal assignment of the HL-A common antigenic determinants in man-mouse somatic cell hybrids.

作者信息

Smith M, Gold P, Shuster J, Tanigaki N, Pressman D

出版信息

J Immunogenet. 1976 Apr;3(2):105-15. doi: 10.1111/j.1744-313x.1976.tb00561.x.

DOI:10.1111/j.1744-313x.1976.tb00561.x

Abstract

In the study presented here, man-mouse somatic cell hybrid clones were examined by means of radioimmunoassays for the presence of both beta2-microglobulin (beta2m) and the HL-A xenoantigenic determinant. In addition, the clones were examined for their karyotype and the expression of enzymes with known chromosomal assignments. The results obtained indicate that the gene coding for the HL-A xenoantigenic determinant is carred on chromosome 6. The data obtained provides a direct demonstration that the gene coding for beta2m segregates independently of that coding for the alloantigenic polypeptide chain of the HL-A molecule, and that the gene coding for beta2m is carried on chromosome 15.

摘要

在本文所介绍的研究中，通过放射免疫测定法对人-小鼠体细胞杂交克隆进行检测，以确定β2-微球蛋白（β2m）和HL-A异种抗原决定簇的存在情况。此外，还对这些克隆的核型以及已知染色体定位的酶的表达进行了检测。所获得的结果表明，编码HL-A异种抗原决定簇的基因位于6号染色体上。所获得的数据直接证明，编码β2m的基因与编码HL-A分子同种异体抗原多肽链的基因独立分离，并且编码β2m的基因位于15号染色体上。