Chandler J A, Sinowatz F, Timms B G, Pierrepoint C G
Cell Tissue Res. 1977 Nov 30;185(1):89-103. doi: 10.1007/BF00226671.
X-ray microanalysis of zinc in ultrathin sections of dog prostate was performed by electron microscope microanalysis using the potassium pyroantimonate method of preparation. Prostates of both mature and immature dogs were examined and the metal was found to be localised primarily in the nucleolus, nuclear chromatin and secretory granules of epithelial cells. Differences in zinc concentrations were observed between mature and immature tissues, particularly in the nuclear chromatin. The metal was also incorporated into epithelial secretions, lysosomes and fibromuscular stroma. Variable binding of zinc to tissue components was revealed by a combination of histochemical precipitation and subcellular analysis.
采用焦锑酸钾制备法，通过电子显微镜微量分析对犬前列腺超薄切片中的锌进行了X射线微量分析。对成熟犬和未成熟犬的前列腺进行了检查，发现该金属主要定位于上皮细胞的核仁、核染色质和分泌颗粒中。观察到成熟组织和未成熟组织之间锌浓度存在差异，特别是在核染色质中。该金属也被整合到上皮分泌物、溶酶体和纤维肌肉基质中。组织化学沉淀和亚细胞分析相结合揭示了锌与组织成分的可变结合。
