波瓦桑病毒：1965年春季传播情况
Powassan virus: vernal spread during 1965.
作者信息
McLean D M, Smith P A, Livingstone S E, Wilson W E, Wilson A G
出版信息
Can Med Assoc J. 1966 Mar 12;94(11):532-6.
Powassan virus was isolated from seven pools of Ixodes cookei ticks removed from groundhogs (Marmota monax) collected near North Bay, Ontario, between May and August 1965, including five pools obtained during spring. Tick pools, each comprising one to nine ticks, contained 2.0 to 5.5 log(10) TCD(50) of virus upon titration in monolayer cultures of primary swine kidney cells. Powassan virus neutralizing antibody prevalence in sera of the current season's groundhogs increased steadily from zero during May to 25% during August but remained relatively unchanged (42% to 58%) in the previous season's groundhogs, thereby confirming that active infection had occurred particularly amongst juvenile groundhogs mainly during spring 1965. Isolation of one strain of Silverwater virus from Haemaphysalis leporis-palustris ticks and detection of neutralizing antibody in three of nine snowshoe hares (Lepus americanus) confirmed the active spread of this agent during 1965.
1965年5月至8月期间，从安大略省北湾附近捕获的土拨鼠（Marmota monax）身上采集的7组库克硬蜱（Ixodes cookei）中分离出波瓦桑病毒，其中包括春季采集的5组。每组蜱虫由1至9只蜱组成，在原代猪肾细胞单层培养物中滴定后，每组蜱虫所含病毒的半数组织培养感染剂量（TCD50）为2.0至5.5 log(10)。当季土拨鼠血清中波瓦桑病毒中和抗体的流行率从5月的零稳步上升至8月的25%，但上一季土拨鼠的中和抗体流行率相对保持不变（42%至58%），从而证实1965年春季尤其是幼年土拨鼠中发生了活跃感染。从沼泽血蜱（Haemaphysalis leporis-palustris）中分离出一株银水病毒，并在9只美洲野兔（Lepus americanus）中的3只体内检测到中和抗体，证实了该病原体在1965年的活跃传播。
Zotero 插件