Daugherty J P, Clapp N K, Zehfus M H, Brock S E
Gan. 1977 Oct;68(5):697-701.
The acid-soluble components of mouse-liver cytosol, prepared at time intervals after intragastric administration of a single carcinogenic dose of 14C-dimethylnitrosamine, were separated by column chromatography. The columns were calibrated with known in vitro metabolites of the nitrosamine, and the elution profiles were compared with those of the mouse-liver system. The results suggest that the 14C-methyl label is transferred to many of the same compounds as identified in vitro, but that numerous other labeled compounds are also present. The possible significance of these metabolites to the pathogenic processes induced by dimethylnitrosamine has yet to be determined.
在经胃内给予单一致癌剂量的14C-二甲基亚硝胺后的不同时间间隔制备小鼠肝脏胞质溶胶的酸溶性成分，通过柱色谱法进行分离。用亚硝胺已知的体外代谢物对柱子进行校准，并将洗脱图谱与小鼠肝脏系统的洗脱图谱进行比较。结果表明，14C-甲基标记转移到了许多与体外鉴定出的相同化合物上，但也存在许多其他标记化合物。这些代谢物对二甲基亚硝胺诱导的致病过程的潜在意义尚待确定。
