Kiss A, Sain B, Csordás-Tòth E, Venetianer P
Gene. 1977 Jul;1(5-6):323-9. doi: 10.1016/0378-1119(77)90037-3.
A new restriction endonuclease has been isolated from Bacillus sphaericus R. The purification procedure includes Bio-Gel filtration, (NH4)2SO4 fractionation and phosphocellulose chromatography. After the phosphocellulose step the enzyme preparation is free of non-specific nucleases. Bsp cleaves double-stranded DNA with the same specificity as Bacillus subtilis (Bsu) and Haemophilus aegyptius (HaeIII) restriction endonucleases, as concluded from digests and double-digests of phiX174 replicative form DNA with Bsu and Bsp. The 5'-terminal nucleotide of the cleavage products was shown to be C. Bacillus sphaericus R produces Bsp in extremely large quantities and the enzyme can be easily purified in high yield.
一种新的限制性内切酶已从球形芽孢杆菌R中分离出来。纯化过程包括生物凝胶过滤、硫酸铵分级分离和磷酸纤维素色谱法。经过磷酸纤维素步骤后，酶制剂不含非特异性核酸酶。从用枯草芽孢杆菌（Bsu）和球形芽孢杆菌（Bsp）对phiX174复制型DNA进行的消化和双酶切结果可以得出，Bsp切割双链DNA的特异性与枯草芽孢杆菌（Bsu）和埃及嗜血杆菌（HaeIII）限制性内切酶相同。切割产物的5'-末端核苷酸显示为C。球形芽孢杆菌R大量产生Bsp，并且该酶可以很容易地以高产率纯化。
