• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Specificity of cleavage by restriction nuclease from Bacillus subtilis.

作者信息

Heininger K, Hörz W, Zachau H G

出版信息

Gene. 1977 Jul;1(5-6):291-303. doi: 10.1016/0378-1119(77)90035-x.

DOI:10.1016/0378-1119(77)90035-x
PMID:22478
Abstract

The restriction nuclease from B. subtilis (Bsu) which cleaves in the middle of the tetra-nucleotide sequence 5'-GGCC-3' 3'-CCGG-5' has been found to decrease its substrate specificity at high nuclease concentrations. There are special conditions, high pH, low ionic strength, and high glycerol content, which strongly enhance splitting with decreased specificity and also lead to splitting of single-stranded DNA. By sequence analyses it is shown that the reduction in specificity of Bsu corresponds to cleavage predominantly at 5'-GC-3' 3'-CG-5' sequences. No comparable change in specificity has been observed in a restriction nuclease from Haemophilus aegyptius (HaeIII), and isoschizomer of Bsu.

摘要

相似文献

1
Specificity of cleavage by restriction nuclease from Bacillus subtilis.
Gene. 1977 Jul;1(5-6):291-303. doi: 10.1016/0378-1119(77)90035-x.
2
Restriction and modification in B. subtilis. Nucleotide sequence recognised by restriction endonuclease R. Bsu R from strain R.枯草芽孢杆菌中的限制与修饰。来自R菌株的限制性内切酶R. Bsu R识别的核苷酸序列。
Mol Gen Genet. 1975 Dec 30;143(1):25-33. doi: 10.1007/BF00269417.
3
A new sequence-specific endonuclease (Bsp) from Bacillus sphaericus.一种来自球形芽孢杆菌的新型序列特异性核酸内切酶（Bsp）。
Gene. 1977 Jul;1(5-6):323-9. doi: 10.1016/0378-1119(77)90037-3.
4
[Specific endonuclease BbvBI from Bacillus brevis].
Bioorg Khim. 1992 Jan;18(1):47-51.
5
Specific cleavage of chromatin by restriction nucleases.限制性核酸酶对染色质的特异性切割。
Nucleic Acids Res. 1976 Nov;3(11):3213-26. doi: 10.1093/nar/3.11.3213.
6
DNA modification induced during infection of Bacillus subtilis by phage phi 3T.噬菌体phi 3T感染枯草芽孢杆菌期间诱导的DNA修饰。
Gene. 1980 Dec;12(1-2):17-24. doi: 10.1016/0378-1119(80)90011-6.
7
Introduction of host-controlled modification and restriction systems of Bacillus subtilis IAM1247 into Bacillus subtilis 168.将枯草芽孢杆菌IAM1247的宿主控制修饰和限制系统引入枯草芽孢杆菌168。
J Bacteriol. 1979 Jul;139(1):308-10. doi: 10.1128/jb.139.1.308-310.1979.
8
Restriction and modification in Bacillus subtilis: two DNA methyltransferases with BsuRI specificity. II. Catalytic properties, substrate specificity, and mode of action.枯草芽孢杆菌中的限制与修饰：两种具有BsuRI特异性的DNA甲基转移酶。II. 催化特性、底物特异性及作用方式。
J Biol Chem. 1981 Sep 10;256(17):9346-51.
9
MspI, an isoschizomer of hpaII which cleaves both unmethylated and methylated hpaII sites.MspI，hpaII的同裂酶，可切割未甲基化和甲基化的hpaII位点。
Nucleic Acids Res. 1978 Sep;5(9):3231-6. doi: 10.1093/nar/5.9.3231.
10
Site-specific cleavage of single-stranded DNA by a Hemophilus restriction endonuclease.一种嗜血杆菌属限制性内切核酸酶对单链DNA的位点特异性切割。
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2555-8. doi: 10.1073/pnas.72.7.2555.

引用本文的文献

1
Type II restriction endonucleases--a historical perspective and more.II型限制性核酸内切酶——历史回顾及更多内容。
Nucleic Acids Res. 2014 Jul;42(12):7489-527. doi: 10.1093/nar/gku447. Epub 2014 May 30.
2
The Fidelity Index provides a systematic quantitation of star activity of DNA restriction endonucleases.保真度指数对DNA限制性核酸内切酶的星号活性进行了系统定量。
Nucleic Acids Res. 2008 May;36(9):e50. doi: 10.1093/nar/gkn182. Epub 2008 Apr 15.
3
Resistance of bacteriophage H1 to restriction and modification by Bacillus subtilis R.
噬菌体H1对枯草芽孢杆菌R的限制和修饰作用的抗性
J Virol. 1983 Jun;46(3):703-8. doi: 10.1128/JVI.46.3.703-708.1983.
4
Nucleotide sequence preference at rat liver and wheat germ type 1 DNA topoisomerase breakage sites in duplex SV40 DNA.双链SV40 DNA中大鼠肝脏和小麦胚芽1型DNA拓扑异构酶断裂位点处的核苷酸序列偏好性。
Nucleic Acids Res. 1984 Apr 11;12(7):3097-114. doi: 10.1093/nar/12.7.3097.
5
Restriction and modification in B. subtilis: effects on transformation and transfection with native and single-stranded DNA.枯草芽孢杆菌中的限制与修饰：对天然DNA和单链DNA转化及转染的影响
Mol Gen Genet. 1980;179(1):103-10. doi: 10.1007/BF00268451.
6
The nucleotide sequences recognized by endonucleases AvaI and AvaII from Anabaena variabilis.可变鱼腥藻中内切核酸酶AvaI和AvaII识别的核苷酸序列。
Biochem J. 1980 Jan 1;185(1):65-75. doi: 10.1042/bj1850065.
7
Selective protection of 5' ... GGCC ... 3' and 5' ... GCNGC ... 3' sequences by the hypermodified oxopyrimidine in Bacillus subtilis bacteriophage SP10 DNA.枯草芽孢杆菌噬菌体SP10 DNA中经超修饰的氧嘧啶对5'... GGCC... 3'和5'... GCNGC... 3'序列的选择性保护
J Virol. 1984 Oct;52(1):47-54. doi: 10.1128/JVI.52.1.47-54.1984.
8
Alteration of the specificity of PvuII restriction endonuclease.PvuII限制性内切酶特异性的改变。
Nucleic Acids Res. 1987 Oct 12;15(19):7677-87. doi: 10.1093/nar/15.19.7677.
9
Purification, properties and specificity of the restriction endonuclease from Bacillus stearothermophilus.嗜热脂肪芽孢杆菌限制性内切酶的纯化、性质及特异性
Biochem J. 1979 Jan 1;177(1):49-62. doi: 10.1042/bj1770049.
10
Isolation of a small DNA fragment carrying the gene for a dihydrofolate reductase from a trimethoprim resistance factor.从甲氧苄啶抗性因子中分离出携带二氢叶酸还原酶基因的小DNA片段。
Mol Gen Genet. 1978 Aug 4;164(1):15-29. doi: 10.1007/BF00267594.