用于将限制酶切末端连接到人工小基因上的两种脱氧核糖十二聚核苷酸的化学合成。

Chemical synthesis of two deoxyribododecanucleotides for the attachment of restriction termini to an artificial minigene.

作者信息

Poonian M S, McComas W W, Nussbaum A L

出版信息

Gene. 1977 Jul;1(5-6):357-72. doi: 10.1016/0378-1119(77)90040-3.

Abstract

In order to permit in vivo cloning of an artificial minigene designed to code for a modified S-peptide, the phosphodiester method for the chemical synthesis of two dodecadeoxyribonucleotides is described. Each of the latter possesses antiparallel complementarity to one of the two minigene strands and to the single-stranded EcoRI-generated end. They can thus serve as cohesive termini ("splints") for polynucleotide ligase joining.

摘要

为了能够在体内克隆一个设计用于编码修饰S肽的人工小基因，本文描述了化学合成两种十二脱氧核糖核苷酸的磷酸二酯法。后者中的每一种都与小基因的两条链之一以及单链EcoRI产生的末端具有反平行互补性。因此，它们可以作为多核苷酸连接酶连接的粘性末端（“夹板”）。