Bongso A, Basrur P K
In Vitro. 1977 Nov;13(11):769-76. doi: 10.1007/BF02627856.
The in vitro fate of bovine fetal fluid cells and the efficiency of fetal sex predication from cultures of these cells are studied using aspirates from live animals and pregnant uteri collected from the slaughterhouse. Over 70 percent of bovine amniotic fluid samples aspirated from pregnant uteri at the time of slaughter yielded cultures adequate for chromosome analysis whereas only 10 percent of allantoic fluid samples produced growth of cells satisfactory for chromosome analysis. Fetal sexing accuracy was 100 percent in all samples studied. Seven readily recognizable cell types were noted in cultures of fetal fluid cells obtained at various stages of gestation. In a majority of cases, the in vitro morphology of cells from both fetal cavities was similar to that observed in primary human amniotic fluid cell cultures.
利用取自活体动物的抽吸物以及从屠宰场收集的怀孕子宫，研究了牛胎儿液体细胞的体外命运以及通过这些细胞培养进行胎儿性别预测的效率。在屠宰时从怀孕子宫抽吸的牛羊水样本中，超过70%产生了足以进行染色体分析的培养物，而尿囊液样本中只有10%产生了对染色体分析而言细胞生长良好的结果。在所研究的所有样本中，胎儿性别鉴定的准确率为100%。在妊娠不同阶段获得的胎儿液体细胞培养物中，观察到了七种易于识别的细胞类型。在大多数情况下，来自两个胎儿腔的细胞的体外形态与在原代人羊水细胞培养物中观察到的相似。
