Bongso T A, Basrur P K
Can J Comp Med. 1977 Jul;41(3):287-93.
Cell contents of foetal fluids obtained separately from amniotic and allantoic cavities of live cows by transvaginal amniocentesis and by aspiration from pregnant uteri immediately after slaughter were studied using Papanicolaou's staining procedure. The samples obtained belonged to cows which were between ten to 24 weeks of gestation. Cell scrapings from foetal and membranous surfaces normally in contact with the foetal fluids were also stained simultaneously with foetal fluid smears by Papanicolaou's method to determine the probable origin of the cells detected in the amniotic and allantoic cavities. The cell types were classified according to morphology and tinctorial patterns and their origin was deduced on the basis of similarity to cell scrapings from the surface layers of foetus and foetal cavities. The evaluation of foetal fluid cells revealed that a majority of cells are of foetal origin and that the cytological features of these cells may be useful in assessing the condition of the foetus.
采用巴氏染色法，对通过经阴道羊膜穿刺术从活牛的羊膜腔和尿囊腔分别获取的羊水细胞内容物，以及在屠宰后立即从怀孕子宫吸出的羊水细胞内容物进行了研究。所获取的样本来自妊娠10至24周的母牛。通常与羊水接触的胎儿和膜表面的细胞刮片，也通过巴氏法与羊水涂片同时染色，以确定在羊膜腔和尿囊腔中检测到的细胞的可能来源。根据形态学和染色模式对细胞类型进行分类，并根据与胎儿和胎儿腔表层细胞刮片的相似性推断其来源。对羊水细胞的评估显示，大多数细胞来源于胎儿，这些细胞的细胞学特征可能有助于评估胎儿的状况。
