人胎盘的胆碱乙酰转移酶
The choline acetyltransferase of human placenta.
作者信息
Morris D
出版信息
Biochem J. 1966 Mar;98(3):754-62. doi: 10.1042/bj0980754.
Abstract
- Various methods for the extraction of choline acetyltransferase (acetyl-CoA-choline O-acetyltransferase, EC 2.3.1.6) from immature human placenta (18-28 weeks of gestation) are described. 2. The crude enzyme was found to be stable at -18 degrees and +4 degrees under a variety of conditions. 3. Purification methods, including ammonium sulphate fractionation, gel filtration on various grades of Sephadex and DEAE-Sephadex fractionation, have yielded a preparation of high specific activity.
摘要
- 本文描述了从未成熟的人胎盘（妊娠18 - 28周）中提取胆碱乙酰转移酶（乙酰辅酶A - 胆碱O - 乙酰转移酶，EC 2.3.1.6）的各种方法。2. 发现粗酶在多种条件下于 - 18℃和 + 4℃时稳定。3. 纯化方法，包括硫酸铵分级分离、不同型号葡聚糖凝胶的凝胶过滤以及二乙氨基乙基葡聚糖凝胶分级分离，已得到具有高比活性的制剂。
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本文引用的文献
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Choline esters in tissue extracts.组织提取物中的胆碱酯类
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Choline acetylase in the placenta of man and other species.人类及其他物种胎盘中的胆碱乙酰化酶。
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A COMPARISON OF THE N-ALKYL GROUP SPECIFICITY OF CHOLINE ACETYLTRANSFERASE FROM DIFFERENT SPECIES.不同物种胆碱乙酰转移酶的N-烷基基团特异性比较
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