一种纯化的大鼠肝脏核酸酶的特性
Properties of a purified rat-liver nuclease.
作者信息
Curtis P J, Smellie R M
出版信息
Biochem J. 1966 Mar;98(3):818-25. doi: 10.1042/bj0980818.
Abstract
- The pH optimum, ionic requirement and heat-stability of a purified liver nuclease have been examined with RNA and denatured DNA as substrates. 2. The enzyme attacked DNA and RNA in an endonucleolytic manner, forming oligonucleotides terminated by 5'-phosphate groups. No clear specificity was found with respect to the bases at the site of cleavage. 3. Comparison of the results obtained with RNA and denatured DNA as substrates suggests that the ribonuclease and deoxyribonuclease activities are associated with the same protein.
摘要
- 以RNA和变性DNA为底物，对纯化的肝脏核酸酶的最适pH值、离子需求和热稳定性进行了研究。2. 该酶以内切核酸酶的方式攻击DNA和RNA，形成以5'-磷酸基团结尾的寡核苷酸。在切割位点的碱基方面未发现明显的特异性。3. 以RNA和变性DNA为底物所获得的结果比较表明，核糖核酸酶和脱氧核糖核酸酶的活性与同一种蛋白质相关。
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Ribonuclease. VI. Partial purification and characterization of the ribonucleases of rat liver mitochondria.核糖核酸酶。VI。大鼠肝脏线粒体核糖核酸酶的部分纯化及特性研究。
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