• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用区带离心法从眼虫中分离完整叶绿体。

Isolation of Intact Chloroplasts from Euglena gracilis by Zonal Centrifugation.

机构信息

Department of Biochemistry and Microbiology (CAES), Rutgers University, New Brunswick, New Jersey 08903.

出版信息

Plant Physiol. 1971 Feb;47(2):217-21. doi: 10.1104/pp.47.2.217.

DOI:10.1104/pp.47.2.217
PMID:16657599
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC365845/
Abstract

Chloroplasts were separated from Euglena gracilis by zonal centrifugation at low speed in density gradients of Ficoll or dextran. The chloroplasts were intact by the criteria of ultrastructure and their content of ribulose diphosphate carboxylase and soluble protein. The chloroplasts also contained ribosomes and ribosomal RNA uncontaminated by the corresponding cytoplasmic particles.

摘要

叶绿体通过在菲可（Ficoll）或葡聚糖密度梯度中低速区带离心从衣藻中分离出来。根据超微结构的标准，叶绿体是完整的，其核酮糖二磷酸羧化酶和可溶性蛋白的含量也是如此。叶绿体还含有核糖体和核糖体 RNA，不受相应细胞质颗粒的污染。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dd7/365845/2b0e5c290789/plntphys00251-0051-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dd7/365845/2bce6bfdfda2/plntphys00251-0050-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dd7/365845/2b0e5c290789/plntphys00251-0051-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dd7/365845/2bce6bfdfda2/plntphys00251-0050-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dd7/365845/2b0e5c290789/plntphys00251-0051-a.jpg

相似文献

1
Isolation of Intact Chloroplasts from Euglena gracilis by Zonal Centrifugation.用区带离心法从眼虫中分离完整叶绿体。
Plant Physiol. 1971 Feb;47(2):217-21. doi: 10.1104/pp.47.2.217.
2
Isolation of Intact Chloroplasts of Euglena gracilis by Isopycnic Sedimentation in Gradients of Silica.用硅石密度梯度等密度沉降法分离眼虫完整叶绿体。
Plant Physiol. 1975 Sep;56(3):399-403. doi: 10.1104/pp.56.3.399.
3
TEMPLATE AND RIBOSOMAL RIBONUCLEIC ACIDS ASSOCIATED WITH THE CHLOROPLASTS AND THE CYTOPLASM OF EUGLENA GRACILIS.与纤细裸藻叶绿体和细胞质相关的模板核糖核酸及核糖体核糖核酸
J Mol Biol. 1964 Dec;10:403-11. doi: 10.1016/s0022-2836(64)80061-9.
4
Isolation of active polyribosomes from the cytoplasm, mitochondria and chloroplasts of Euglena gracilis.从纤细裸藻的细胞质、线粒体和叶绿体中分离活性多核糖体。
Biochem J. 1972 Jun;128(2):353-65. doi: 10.1042/bj1280353.
5
Synthesis of Proteins by Isolated Euglena gracilis Chloroplasts.用分离的眼虫(Euglena gracilis)叶绿体合成蛋白质。
Plant Physiol. 1976 Dec;58(6):719-21. doi: 10.1104/pp.58.6.719.
6
Ultrastructure and physical characteristics of proplastids and chloroplasts isolated by zonal centrifugation from Euglena gracilis var. bacillaris.通过区带离心从纤细裸藻杆状变种中分离出的前质体和叶绿体的超微结构及物理特性。
Eur J Cell Biol. 1980 Aug;21(3):305-12.
7
Chloroplast ribosomes in the proplastids of Euglena gracilis.纤细裸藻前质体中的叶绿体核糖体。
Biochim Biophys Acta. 1971 Apr 8;232(4):696-704. doi: 10.1016/0005-2787(71)90761-1.
8
Isolation and characterization of Euglena gracilis cytoplasmic and chloroplast ribosomes and their ribosomal RNA components.纤细裸藻胞质核糖体和叶绿体核糖体及其核糖体RNA组分的分离与鉴定
Biochim Biophys Acta. 1969 Oct 22;190(2):368-80. doi: 10.1016/0005-2787(69)90087-2.
9
Studies on the isolation and purification of chloroplasts from Euglena gracilis.从眼虫中分离和纯化叶绿体的研究。
Planta. 1972 Dec;107(4):351-67. doi: 10.1007/BF00386396.
10
The isolation of Euglena gracilis chloroplasts uncontaminated by nuclear DNA.分离未受核DNA污染的纤细裸藻叶绿体。
Biochim Biophys Acta. 1972 Jan 18;259(1):1-4. doi: 10.1016/0005-2787(72)90467-4.

引用本文的文献

1
Studies on the isolation and purification of chloroplasts from Euglena gracilis.从眼虫中分离和纯化叶绿体的研究。
Planta. 1972 Dec;107(4):351-67. doi: 10.1007/BF00386396.
2
Photosynthetic activity of isolated chloroplasts from Euglena gracilis.来自眼虫的分离叶绿体的光合作用活性。
Planta. 1982 Mar;154(1):70-5. doi: 10.1007/BF00385499.
3
Fractionation and identification of Euglena gracilis cytoplasmic and chloroplastic tRNAs and mapping of tRNA genes on chloroplast DNA.小球藻细胞质和叶绿体 tRNA 的分离与鉴定及叶绿体 DNA 上 tRNA 基因的定位。

本文引用的文献

1
Early effects of illumination on the activity of some photosynthetic enzymes.光照对一些光合酶活性的早期影响。
Plant Physiol. 1967 Jan;42(1):1-5. doi: 10.1104/pp.42.1.1.
2
Euglena gracilis, A Test Organism for Study of Zinc.纤细裸藻，一种用于锌研究的试验生物。
Plant Physiol. 1962 May;37(3):428-33. doi: 10.1104/pp.37.3.428.
3
A study of fixation for electron microscopy.电子显微镜固定研究。
Curr Genet. 1982 Oct;6(1):63-9. doi: 10.1007/BF00397643.
4
Preparation of chloroplasts from euglena highly active in protein synthesis.从 Euglena 中制备高度活跃于蛋白质合成的叶绿体。
Plant Physiol. 1980 Aug;66(2):291-4. doi: 10.1104/pp.66.2.291.
5
Isolation of Intact Chloroplasts of Euglena gracilis by Isopycnic Sedimentation in Gradients of Silica.用硅石密度梯度等密度沉降法分离眼虫完整叶绿体。
Plant Physiol. 1975 Sep;56(3):399-403. doi: 10.1104/pp.56.3.399.
6
Photosynthetic activity of spinach chloroplasts after isopycnic centrifugation in gradients of silica.在二氧化硅梯度等密度离心后菠菜叶绿体的光合作用活性。
Plant Physiol. 1974 Oct;54(4):532-4. doi: 10.1104/pp.54.4.532.
7
The chloroplast and cytoplasmic ribosomes of euglena: I. Stability of chloroplast ribosomes prepared by an improved procedure.眼虫的叶绿体核糖体和细胞质核糖体：I. 用改进方法制备的叶绿体核糖体的稳定性。
Plant Physiol. 1974 Apr;53(4):533-42. doi: 10.1104/pp.53.4.533.
8
Nucleotide sequence of a Euglena gracilis chloroplast genome region coding for the elongation factor Tu; evidence for a spliced mRNA.纤细裸藻叶绿体基因组中编码延伸因子Tu的区域的核苷酸序列；剪接mRNA的证据。
Nucleic Acids Res. 1983 Sep 10;11(17):5877-92. doi: 10.1093/nar/11.17.5877.
9
Isolation of active polyribosomes from the cytoplasm, mitochondria and chloroplasts of Euglena gracilis.从纤细裸藻的细胞质、线粒体和叶绿体中分离活性多核糖体。
Biochem J. 1972 Jun;128(2):353-65. doi: 10.1042/bj1280353.
10
The sites of transcription and translation for Euglena chloroplastic aminoacyl-tRNA synthetases.眼虫叶绿体氨酰-tRNA合成酶的转录和翻译位点。
Proc Natl Acad Sci U S A. 1974 May;71(5):1910-4. doi: 10.1073/pnas.71.5.1910.
J Exp Med. 1952 Mar;95(3):285-98. doi: 10.1084/jem.95.3.285.
4
A MEMBRANE METHOD FOR DETERMINATION OF TOTAL PROTEIN IN DILUTE ALGAL SUSPENSIONS.一种用于测定稀藻悬浮液中总蛋白的膜法
Anal Biochem. 1965 Aug;12:213-8. doi: 10.1016/0003-2697(65)90084-9.
5
THE PROTEIN-SYNTHESIZING SYSTEMS FROM THE CYTOPLASM AND THE CHLOROPLASTS OF EUGLENA GRACILIS.纤细裸藻细胞质和叶绿体的蛋白质合成系统
J Mol Biol. 1964 Dec;10:392-402. doi: 10.1016/s0022-2836(64)80060-7.
6
THE ISOLATION OF STRUCTURALLY INTACT CHLOROPLASTS.结构完整的叶绿体的分离
Biochim Biophys Acta. 1964 May 25;79:637-9. doi: 10.1016/0926-6577(64)90235-9.
7
The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.在电子显微镜检查中，将高pH值的柠檬酸铅用作电子不透明染色剂。
J Cell Biol. 1963 Apr;17(1):208-12. doi: 10.1083/jcb.17.1.208.
8
A comment on the spectrophotometric determination of chlorophyll.关于叶绿素分光光度测定法的一则评论
Biochim Biophys Acta. 1961 Sep 30;52:576-8. doi: 10.1016/0006-3002(61)90418-8.
9
Sedimentation rates of plastids in an analytical zonal rotor.分析区带转子中质体的沉降速率。
Biochim Biophys Acta. 1967 Nov 28;148(2):531-8. doi: 10.1016/0304-4165(67)90152-3.
10
Characterization of macromolecules by constant velocity sedimentation.通过等速沉降法对大分子进行表征。
Nature. 1967 Jul 22;215(5099):360-3. doi: 10.1038/215360a0.