Bhat U K, Yunker C E
J Parasitol. 1977 Dec;63(6):1092-8.
Establishment of a continuous cell line (RML-14) from embryonic tissues of the tick Dermacentor parumapertus Neumann is reported. The culture medium employed consisted of a combination (2:1) of Eagle's and L-15 (Leibovitz) media supplemented with 20% fetal bovine serum, 10% tryptose phosphate broth, and 0.1% bovine plasma albumin. At the 8th passage, 99% of dividing cells had the female chromosome complement, among which more than 70% had a diploid chromosome number of 22. At the 13th passage, cell population showed approximately a 3-fold increase during the first 8 days of culture. As of December 1976, had been subcultured 40 times.
据报道，已从微小牛蜱（Dermacentor parumapertus Neumann）的胚胎组织中建立了一个连续细胞系（RML - 14）。所使用的培养基由伊格尔培养基和L - 15（莱博维茨）培养基按2:1的比例混合而成，并添加了20%的胎牛血清、10%的胰蛋白胨磷酸盐肉汤和0.1%的牛血浆白蛋白。在第8代时，99%的分裂细胞具有雌性染色体组成，其中70%以上具有22条二倍体染色体数。在第13代时，细胞群体在培养的前8天中显示出约3倍的增长。截至1976年12月，已传代40次。
