Moulton J E
Am J Vet Res. 1978 Sep;39(9):1558-64.
Primary cell cultures were prepared from preimaginal bodies from the nymphal ticks, Rhipicephalus sanguineus, Dermacentor andersoni, and Amblyomma maculatum, and from the hemocytes of late-stage nymphal and adult ticks, Ornithodoros coriaceus. The dissection methods for obtaining preimaginal bodies and hemocytes for culture are described. A culture mediu, designated RML 375, was used for both culture methods. Primary cultures were established with minimal contamination. Preimaginal body cultures may be maintained for 2 to 8 months or longer and hemocyte cultures for 1 to 9 months. Cultured cells form incomplete monolayers and tend to grow in clusters. Subculturing these cells is difficult, although A maculatum have been sucessfully subcultured several times. A brief review of arthropod tissue culturing is presented and potential applications of the method are discussed.
原代细胞培养取自血红扇头蜱、安德逊革蜱和斑点钝眼蜱若虫期的预成虫体，以及科氏钝缘蜱若虫后期和成虫期的血细胞。文中描述了获取用于培养的预成虫体和血细胞的解剖方法。一种名为RML 375的培养基用于两种培养方法。原代培养建立时污染极少。预成虫体培养物可维持2至8个月或更长时间，血细胞培养物可维持1至9个月。培养的细胞形成不完整的单层，倾向于聚集成簇生长。尽管斑点钝眼蜱已成功传代培养数次，但这些细胞的传代培养仍很困难。本文简要回顾了节肢动物组织培养，并讨论了该方法的潜在应用。
