Mota I
Immunology. 1966 Aug;11(2):137-40.
Normal and splenectomized rats were injected by the intravenous route with small amounts of soluble antigen (egg albumin) or particulate antigen (toad red cells) plus vaccine and bled at various times afterwards. The reaginic antibody was assayed by passive cutaneous anaphylaxis using a 72-hour period between antibody and antigen injection and the haemolytic antibody by determining the highest dilution of serum giving complete lysis in presence of complement. It was observed that although splenectomized animals responded with a very much diminished production of haemolytic antibody their production of reaginic antibody was very similar to that of the non-splenectomized animals. It is suggested that the spleen plays little or no role in the production of rat reaginic antibody.
正常大鼠和脾切除大鼠通过静脉途径注射少量可溶性抗原（卵清蛋白）或颗粒性抗原（蟾蜍红细胞）加疫苗，之后在不同时间采血。使用抗体和抗原注射间隔72小时的被动皮肤过敏反应来检测反应素抗体，通过测定在补体存在下血清能产生完全溶血的最高稀释度来检测溶血抗体。观察到，虽然脾切除动物产生的溶血抗体量大大减少，但其反应素抗体的产生与未脾切除动物非常相似。提示脾脏在大鼠反应素抗体的产生中作用很小或没有作用。
