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猪神经垂体素的制备、性质以及钙对激素-神经垂体素复合物的影响。

The preparation and properties of porcine neurophysin and the influence of calcium on the hormone-neurophysin complex.

作者信息

Ginsburg M, Jayasena K, Thomas P J

出版信息

J Physiol. 1966 May;184(2):387-401. doi: 10.1113/jphysiol.1966.sp007921.

DOI:10.1113/jphysiol.1966.sp007921
PMID:5921837
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1357567/
Abstract
  1. The preparation of a hormone-binding protein fraction (neurophysin) from porcine neurohypophyses is described.2. It was shown by gel-filtration that the protein (which sedimented as a single component on analytical ultracentrifugation) forms complexes with oxytocin and lysine vasopressin. The maximum capacity of porcine neurophysin to bind oxytocin and lysine vasopressin was estimated from the results of dialysis experiments as 232 u. oxytocin and 48 u. lysine vasopressin/mg protein.3. Binding of oxytocin and lysine vasopressin by neurophysin was completely inhibited in the presence of calcium in concentrations greater than 10(-6)M but is unaffected at less than 10(-7)M-Ca. The concentration of calcium required to inhibit binding did not appear to be dependent on hormone or protein concentrations.4. No evidence was obtained that calcium was bound by neurophysin or by the peptide hormones either alone or in combination.5. These results are discussed in the light of the hypothesis that release of the hormone from the neurohypophysis is mediated by calcium.
摘要

  1. 本文描述了从猪神经垂体中制备激素结合蛋白组分（神经垂体素）的方法。

  2. 通过凝胶过滤显示，该蛋白（在分析超速离心中作为单一成分沉降）与催产素和赖氨酸加压素形成复合物。根据透析实验结果估计，猪神经垂体素结合催产素和赖氨酸加压素的最大能力分别为每毫克蛋白232单位催产素和48单位赖氨酸加压素。

  3. 当钙浓度大于10^(-6)M时，神经垂体素对催产素和赖氨酸加压素的结合被完全抑制，但在低于10^(-7)M - Ca时不受影响。抑制结合所需的钙浓度似乎不依赖于激素或蛋白浓度。

  4. 没有证据表明钙单独或与肽类激素结合时会与神经垂体素结合。

  5. 根据激素从神经垂体释放由钙介导的假说对这些结果进行了讨论。

相似文献

1
The preparation and properties of porcine neurophysin and the influence of calcium on the hormone-neurophysin complex.猪神经垂体素的制备、性质以及钙对激素-神经垂体素复合物的影响。
J Physiol. 1966 May;184(2):387-401. doi: 10.1113/jphysiol.1966.sp007921.
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THE PREPARATION OF BOVINE NEUROPHYSIN AND THE ESTIMATION OF ITS MAXIMUM CAPACITY TO BIND OXYTOCIN AND ARGININE VASOPRESSIN.牛神经垂体素的制备及其结合催产素和精氨酸加压素最大能力的测定
J Endocrinol. 1965 May;32:187-98. doi: 10.1677/joe.0.0320187.
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Biochem J. 1973 Mar;132(3):361-71. doi: 10.1042/bj1320361.
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The distribution of proteins that bind neurohypophysial hormones.结合神经垂体激素的蛋白质的分布。
J Physiol. 1968 Jul;197(1):65-76. doi: 10.1113/jphysiol.1968.sp008546.
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The molecular dimensions of porcine neurophysin and some thermodynamic parameters of the reaction with lysine vasopressin.猪神经垂体素的分子尺寸及与赖氨酸加压素反应的一些热力学参数。
J Physiol. 1969 Mar;201(1):181-99. doi: 10.1113/jphysiol.1969.sp008750.
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Inhibition by L-cystine of the binding of 8-lysine vasopressin by porcine neurophysin.L-胱氨酸对猪神经垂体素结合8-赖氨酸加压素的抑制作用。
Biochem J. 1966 Jul;100(1):9C-10C.
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The isolation of the native hormone-binding proteins from bovine pituitary posterior lobes. Crystallization of neurophysin-I and-II as complexes with [8-arginine]-vasopressin.从牛垂体后叶中分离天然激素结合蛋白。神经垂体素-I和-II与[8-精氨酸]-加压素形成复合物的结晶。
Biochem J. 1968 Jan;106(2):557-64. doi: 10.1042/bj1060557.
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Isolation of a third bovine neurophysin.第三种牛神经垂体素的分离
Biochem J. 1969 Nov;115(3):473-9. doi: 10.1042/bj1150473.
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The isolation of three neurophysins from porcine posterior pituitary lobes.从猪垂体后叶中分离出三种神经垂体素。
Biochem J. 1970 Mar;116(5):899-909. doi: 10.1042/bj1160899.
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Polymerizing equilibria in neurophysin.神经垂体素中的聚合平衡。
J Physiol. 1969 Dec;205(3):635-46. doi: 10.1113/jphysiol.1969.sp008987.

引用本文的文献

1
Release of neurohypophysial hormones in vitro.神经垂体激素的体外释放
J Physiol. 1967 May;190(1):171-87. doi: 10.1113/jphysiol.1967.sp008200.
2
The molecular dimensions of porcine neurophysin and some thermodynamic parameters of the reaction with lysine vasopressin.猪神经垂体素的分子尺寸及与赖氨酸加压素反应的一些热力学参数。
J Physiol. 1969 Mar;201(1):181-99. doi: 10.1113/jphysiol.1969.sp008750.
3
The distribution of proteins that bind neurohypophysial hormones.结合神经垂体激素的蛋白质的分布。
J Physiol. 1968 Jul;197(1):65-76. doi: 10.1113/jphysiol.1968.sp008546.
4
The neurophysins of the pig.猪的神经垂体素
Biochem J. 1967 Nov;105(2):34C-36C. doi: 10.1042/bj1050034c.
5
[Fluorescence-microscopic detection of arginine in the neurosecretory material of some mammalian species].[某些哺乳动物物种神经分泌物质中精氨酸的荧光显微镜检测]
Histochemie. 1971;25(2):152-62.
6
Polymerizing equilibria in neurophysin.神经垂体素中的聚合平衡。
J Physiol. 1969 Dec;205(3):635-46. doi: 10.1113/jphysiol.1969.sp008987.
7
Optical activity of bovine neurophysins and their peptide complexes in the near ultraviolet.牛神经垂体素及其肽复合物在近紫外区的旋光性。
Proc Natl Acad Sci U S A. 1970 Oct;67(2):493-500. doi: 10.1073/pnas.67.2.493.
8
Release in vitro of neurohypophysial proteins from neural lobe tissue slices and from isolated neurosecretory granules of the rat.大鼠神经叶组织切片及分离的神经分泌颗粒中神经垂体蛋白的体外释放
Z Zellforsch Mikrosk Anat. 1972;129(1):114-39. doi: 10.1007/BF00307114.
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The occurrence of antigen reacting with antibody to porcine neurophysin.与抗猪神经垂体素抗体发生反应的抗原的出现。
J Physiol. 1968 Jul;197(1):53-63. doi: 10.1113/jphysiol.1968.sp008545.
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Subcellular distribution of neurophysin in rats subjected to haemorrhage, salt-loading and lactation and in rats with hereditary diabetes insipidus (Brattleboro strain).失血性、盐负荷性及哺乳期大鼠以及遗传性尿崩症（布拉特洛维菌株）大鼠中神经垂体素的亚细胞分布。
Z Zellforsch Mikrosk Anat. 1973;140(3):413-24. doi: 10.1007/BF00307027.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
THE PREPARATION OF BOVINE NEUROPHYSIN AND THE ESTIMATION OF ITS MAXIMUM CAPACITY TO BIND OXYTOCIN AND ARGININE VASOPRESSIN.牛神经垂体素的制备及其结合催产素和精氨酸加压素最大能力的测定
J Endocrinol. 1965 May;32:187-98. doi: 10.1677/joe.0.0320187.
3
THE EFFECTS OF CALCIUM ON PROTEIN-BINDING AND METABOLISM OF ARGININE VASOPRESSIN IN RATS.钙对大鼠精氨酸加压素蛋白结合及代谢的影响
J Endocrinol. 1965 May;32:141-51. doi: 10.1677/joe.0.0320141.
4
BINDING OF VASOPRESSIN AND OXYTOCIN TO PROTEIN IN EXTRACTS OF BOVINE AND RABBIT NEUROHYPOPHYSES.血管加压素和催产素与牛和兔神经垂体提取物中蛋白质的结合
J Endocrinol. 1964 Aug;30:131-45. doi: 10.1677/joe.0.0300131.
5
STIMULUS-SECRETION COUPLING IN A NEUROSECRETORY ORGAN: THE ROLE OF CALCIUM IN THE RELEASE OF VASOPRESSIN FROM THE NEUROHYPOPHYSIS.神经分泌器官中的刺激-分泌偶联：钙在神经垂体释放血管加压素中的作用。
J Physiol. 1964 Jul;172(1):1-18. doi: 10.1113/jphysiol.1964.sp007399.
6
CALCIUM MOVEMENT IN THE NEUROHYPOPHYSIS OF THE RAT AND ITS RELATION TO THE RELEASE OF VASOPRESSIN.大鼠神经垂体中的钙运动及其与血管加压素释放的关系
J Physiol. 1964 Jul;172(1):19-30. doi: 10.1113/jphysiol.1964.sp007400.
7
THE DEPENDENCE OF CONTRACTION AND RELAXATION OF MUSCLE FIBRES FROM THE CRAB MAIA SQUINADO ON THE INTERNAL CONCENTRATION OF FREE CALCIUM IONS.螃蟹（黄道蟹）肌纤维收缩与舒张对游离钙离子胞内浓度的依赖性
Biochim Biophys Acta. 1964 May 25;79:581-91. doi: 10.1016/0926-6577(64)90224-4.
8
The application of four methods for assessing protein homogeneity to crystalline beta-lactoglobulin: an anomaly in phase rule solubility tests.四种评估蛋白质均一性的方法在结晶β-乳球蛋白中的应用：相律溶解度测试中的一个异常现象。
Biochem J. 1954 Sep;58(1):31-8. doi: 10.1042/bj0580031.