Grut W, Edwards J, Evans E J
J Microsc. 1977 Aug;110(3):271-5. doi: 10.1111/j.1365-2818.1977.tb00037.x.
As part of a larger project aimed at claryfying some of the uncertainties that exist concerning the structural basis for an explanation of the mechanical behaviour of elastin, an examination was made of purified porcine aortic elastin under the scanning electron microscopy. In order to avoid structural changes caused by impregnation with organic solvents, specimens were prepared directly from their water-saturated state by either air-drying of freeze-drying processes. Comparison of the results obtained by these techniques demonstrates that freeze-drying of elastin has the advantage of enhancing fine detail, minimizing distortion and reducing specimen shrinkage.
作为一个更大项目的一部分，该项目旨在澄清关于弹性蛋白力学行为解释的结构基础方面存在的一些不确定性，我们在扫描电子显微镜下对纯化的猪主动脉弹性蛋白进行了检查。为了避免因用有机溶剂浸渍而导致结构变化，通过空气干燥或冷冻干燥过程直接从水饱和状态制备标本。对这些技术获得的结果进行比较表明，弹性蛋白的冷冻干燥具有增强细节、最小化变形和减少标本收缩的优点。
