Hrushovetz S B, Mulder R J
Can Med Assoc J. 1966 Nov 12;95(20):1019-23.
Employing L and HeLa cells grown as a monolayer on coverslips in Leighton tubes, 19 human effusions were screened for the presence of growth-promoting factors. The screening test consisted in recording from microscopic studies the percentage of cells in division after incubation for 36 hours in the test medium, with the last 12 hours of incubation in the presence of a mitostatic agent. The effusions were diluted to one-third strength with Hanks' balanced salt solution and were tested in triplicate; a minimum of 500 cells were counted for each replicate. All effusions possessed growth-promoting activity. The results suggested that effusions from patients with malignant disease do not differ in growth-promoting activity in cell culture from effusions obtained from patients with non-malignant disorders.
利用在莱顿管盖玻片上单层生长的L细胞和HeLa细胞，对19份人体积液进行了生长促进因子存在情况的筛查。筛查试验包括通过显微镜研究记录在试验培养基中孵育36小时后处于分裂期的细胞百分比，其中最后12小时的孵育是在有丝分裂抑制剂存在的情况下进行的。将积液用汉克斯平衡盐溶液稀释至三分之一浓度，并进行三次重复测试；每个重复样本至少计数500个细胞。所有积液都具有生长促进活性。结果表明，恶性疾病患者的积液在细胞培养中的生长促进活性与非恶性疾病患者的积液没有差异。
