Hayes J A, Christensen T G, Snider G L
Lab Anim Sci. 1977 Oct;27(5 Pt 2):762-70.
Male hamster weighing 100-140 g were exposed to 50-500 microgram purified elastrase per 100 g body weight by intratracheal injection. The hamster were killed at intervals up to 12 months after treatment, and their lungs were examined grossly and microscopically. At 8 days after treatment, the lungs appeared to have a greater volume than the controls. At 16 days, the lungs remained inflated when the pleural cavity was opened. Alveolar size was increased in proportion to the dose of elastase. From 16 days onward, the emphysematous lesion was well established. The number of goblet cells in the bronchi also increased. Counts of goblet cells as a proportion of all bronchial lining cells showed that goblet cells more than doubled in number in elastase treated hamsters as compared with controls. The mechanism for the two lesions caused by elastase was not clear. It was concluded that the elastase treated hamster is a useful model for studies of the pathogenesis of chronic bronchitis and emphysema in man.
体重100 - 140克的雄性仓鼠通过气管内注射，每100克体重接受50 - 500微克纯化弹性蛋白酶。在治疗后长达12个月的时间间隔内处死仓鼠，并对其肺部进行大体和显微镜检查。治疗后8天，肺部体积似乎比对照组大。16天时，打开胸腔时肺部仍处于膨胀状态。肺泡大小与弹性蛋白酶剂量成比例增加。从16天起，肺气肿病变已充分形成。支气管中杯状细胞的数量也增加了。杯状细胞数量占所有支气管内衬细胞的比例计数显示，与对照组相比，弹性蛋白酶处理的仓鼠中杯状细胞数量增加了一倍多。弹性蛋白酶引起这两种病变的机制尚不清楚。得出的结论是，弹性蛋白酶处理的仓鼠是研究人类慢性支气管炎和肺气肿发病机制的有用模型。
