Gralla E J, Agostinelli A G, Russfield A B, Folsch E
Lab Anim Sci. 1977 Oct;27(5 Pt 2):866-78.
Peripheral blood and thoracic duct lymph from normal dogs and dogs with a leukemic malignant lymphoma were compared for total and differential leukocyte count and lymph flow rate. Except for higher numbers of circulating atypical lymphocytes, the blood leukocyte count as well as the lymph cell count and flow rate were similar in both groups. Lymph cell differential pattersn in lymphoma dogs had higher numbers of lymphoblasts and minor cell types, plasmacytes, monocytes, and reticulum cells, plus degenerating and mitotic cells. There was a five-fold increase in the lymph leukocyte count shortly after irradiation in two lymphomatous dosg. Stained preparations of this lymph showed signs of 100% cell mortality. It was concluded that the thoracic duct lymph is a practical source of normal and cancerous lymphoid cells, and that the lymph and cells collected from dogs with malignant lymphoma are excellent models for certain studies of spontaneous neoplasia.
对正常犬和患有白血病性恶性淋巴瘤的犬的外周血和胸导管淋巴进行了白细胞总数、分类计数及淋巴液流速的比较。除了循环中的非典型淋巴细胞数量较多外，两组的血液白细胞计数以及淋巴细胞计数和流速相似。淋巴瘤犬的淋巴细胞分类模式显示，成淋巴细胞以及少量细胞类型（浆细胞、单核细胞和网状细胞）、退变细胞和有丝分裂细胞数量较多。两只淋巴瘤犬在照射后不久，淋巴白细胞计数增加了五倍。这种淋巴的染色制剂显示出100%细胞死亡的迹象。得出的结论是，胸导管淋巴是正常和癌性淋巴细胞的一个实用来源，并且从患有恶性淋巴瘤的犬收集的淋巴和细胞是某些自发性肿瘤研究的优秀模型。
