Spencer J, Hall J G
Immunology. 1984 May;52(1):7-15.
By cannulating the efferent duct of the caudal mediastinal lymph node in sheep, lymph from the lower respiratory tract was collected under physiological conditions for several days. In 18 such preparations the flow rate varied from 4 to 12 ml/hr between individuals and the lymphocyte count between 4000 and 117,000/mm3. The protein content of the lymph plasma averaged nearly 60% of that of the blood, and this indication of the high permeability of the capillary bed of the lungs was confirmed by measuring the time taken for intravenous doses of 125I-albumin to equilibrate between the blood and mediastinal lymph plasma. The concentration of immunoglobulin A was higher in the mediastinal lymph than in blood serum, while the reverse was true of the concentrations of IgG1, IgG2, and IgM. This evidence for the local production of IgA by the intra thoracic lymphoid tissue was supported by the demonstration by immunoperoxidase techniques of IgA-containing plasma cells in sections cut from the caudal mediastinal nodes, and of IgA-containing immunoblasts in the lymph.
通过对绵羊尾纵隔淋巴结的输出管进行插管，在生理条件下连续数天收集来自下呼吸道的淋巴液。在18个这样的样本中，个体间的流速在4至12毫升/小时之间，淋巴细胞计数在4000至117,000/mm³之间。淋巴血浆中的蛋白质含量平均接近血液的60%，通过测量静脉注射的125I-白蛋白在血液和纵隔淋巴血浆之间达到平衡所需的时间，证实了肺毛细血管床具有高通透性。纵隔淋巴中免疫球蛋白A的浓度高于血清，而IgG1、IgG2和IgM的浓度则相反。尾纵隔淋巴结切片中含IgA浆细胞以及淋巴中含IgA免疫母细胞的免疫过氧化物酶技术证明，支持了胸腔内淋巴组织局部产生IgA的这一证据。
