一种用于测定血清胆固醇的简化比色微量法。
A simplified colorimetric micromethod for determination of serum cholesterol.
作者信息
Bhandaru R R, Srinivasan S R, Pargaonkar P S, Berenson G S
出版信息
Lipids. 1977 Dec;12(12):1078-80. doi: 10.1007/BF02533337.
A simple and rapid micromethod is described for quantitation of cholesterol in 5-10 microliter of serum using uranyl acetate to deproteinize the serum and o-phthalaldehyde reagent to develop color. Independent analyses of serum samples by the present method and by two reference methods, Abell-Kendall and automated colorimetric method, indicated accuracy of the micromethod (r = 0.99). Use of capillary blood specimens makes this procedure applicable for screening infants and small children.
本文描述了一种简单快速的微量方法，用于定量测定5 - 10微升血清中的胆固醇。该方法使用醋酸铀酰使血清脱蛋白，并使用邻苯二甲醛试剂显色。通过本方法与两种参考方法（阿贝尔 - 肯德尔法和自动比色法）对血清样本进行独立分析，结果表明该微量方法具有准确性（r = 0.99）。使用毛细血管血标本使得该程序适用于筛查婴幼儿。
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