Schulz A
Microsc Acta. 1977 Nov;80(1):7-18.
Undecalcified preparation of bone tissue for electron microscopic investigation depends on an adequate embedding method. A low viscosity resin proved to comply with the particular conditions as there is good infiltration of specimen by the monomer medium and also sufficient hardness of the polymerization product. Both properties stabilize the transition of soft tissue to mineralized bone surface; thus reproducible results of good quality are obtained in ultra-thin sectioning. A special preparation technique of human bone biopsies for simultaneous light and electron microscopic investigation has been developed. The method is based on experiences with approximately 300 iliac crest biopsies in metabolic and endocrine bone disorders.
用于电子显微镜研究的骨组织脱钙制剂取决于适当的包埋方法。低粘度树脂被证明符合特定条件，因为单体介质对标本有良好的浸润性，且聚合产物有足够的硬度。这两种特性稳定了软组织向矿化骨表面的转变；因此，在超薄切片中可获得质量良好的可重复结果。已经开发出一种用于同时进行光镜和电镜研究的人骨活检特殊制备技术。该方法基于在代谢性和内分泌性骨疾病中对约300例髂嵴活检的经验。
