Schulz A
Beitr Pathol. 1975 Dec;156(3):280-8.
The preparation of undecalcified bone tissue for electron microscopic investigation depends on the quality of the embedding medium. Penetration of bone tissue is improved by a low viscosity of the monomer plastic material. Hardness of polymerized embedding medium should be similar to bone tissue, but a good cutting quality must be maintained. A low viscosity resin proposed by Spurr (1969) proved to be in compliance with these conditions.
The embedding medium is composed of four components: vinylcyclohexenedioxide (ERL, 4206), polyglycolepoxide (DER 736), nonenyl succinic anhydride (NAS) and dimethylaminoethanol (DMAE). The most favourable molar ratio between anhydride (NSA) and epoxide (ERL 4206 and DER 736) was A : E = 0.81 : I. Hardness and brittleness of polymerized epoxy resin can additionally be influenced by varying the amounts of both epoxy components in the final mixture. Human iliac crest trabecular bone as well as rat bone tissue were embedded to test the quality of the medium. Resutls: Ultra-thin sections were performed at low cutting speed (0.5 mm/sec) using diamond knives. Artificial alterations of bone surfaces did hardly occur due to a good penetration of the bone tissue. The special hardness of the embedding medium stabilized the tissue, thus chattering of the transition from osteoid to mineralized bone could be markedly reduced.
用于电子显微镜研究的未脱钙骨组织的制备取决于包埋介质的质量。单体塑料材料的低粘度可提高骨组织的渗透性。聚合包埋介质的硬度应与骨组织相似，但必须保持良好的切割质量。Spurr（1969年）提出的一种低粘度树脂被证明符合这些条件。
包埋介质由四种成分组成：乙烯基环己烯二氧化物（ERL，4206）、聚环氧乙烷（DER 736）、壬烯基琥珀酸酐（NAS）和二甲基氨基乙醇（DMAE）。酸酐（NSA）与环氧化物（ERL 4206和DER 736）之间最适宜的摩尔比为A:E = 0.81:1。通过改变最终混合物中两种环氧成分的含量，可进一步影响聚合环氧树脂的硬度和脆性。用人髂嵴小梁骨以及大鼠骨组织进行包埋以测试该介质的质量。结果：使用金刚石刀以低切割速度（0.5毫米/秒）制作超薄切片。由于骨组织的良好渗透性，骨表面几乎未出现人为改变。包埋介质的特殊硬度使组织稳定，因此可显著减少从类骨质到矿化骨过渡处的颤动。
