Chapman C J, Nugent N A, Schreiber R W
Plant Physiol. 1966 Apr;41(4):589-92. doi: 10.1104/pp.41.4.589.
Radioautographic and radiochemical techniques were used to establish the presence of replicating DNA in the chloroplasts of Acetabularia mediterranea. These techniques also demonstrated that these chloroplasts synthesize RNA. It was found that label from thymine was also incorporated into DNA and RNA in these chloroplasts. With the establishment of protein and nucleic acid synthesis in Acetabularia chloroplasts, it is clear that these chloroplasts carry out those metabolic processes which are most characteristic of autonomous cells.
采用放射自显影和放射化学技术来确定地中海伞藻叶绿体中存在正在复制的DNA。这些技术还表明这些叶绿体能够合成RNA。研究发现,胸腺嘧啶的标记物也会掺入这些叶绿体的DNA和RNA中。随着伞藻叶绿体中蛋白质和核酸合成的确立,很明显这些叶绿体能够进行那些自主细胞最具特征的代谢过程。