Tymms M J, Schweiger H G
Proc Natl Acad Sci U S A. 1985 Mar;82(6):1706-10. doi: 10.1073/pnas.82.6.1706.
A purified chloroplast fraction was prepared from caps of the giant unicellular green alga Acetabularia mediterranea (strain 17). High molecular weight DNA obtained from these chloroplasts contains at least five copies of a 10-kilobase-pair (kbp) sequence tandemly arranged. This unique sequence is present in DNA from chloroplasts of all stages of the life cycle examined. A chloroplast rDNA clone from mustard hybridized with some restriction fragments from Acetabularia chloroplast DNA but not with the repeated sequence. An 8-kbp EcoRI-Pst I fragment of the repeated sequence was cloned into pBR322 and used as a hybridization probe. No homology was found between the cloned 8-kbp sequence and chloroplast DNA from related species Acetabularia crenulata or chloroplast DNA from spinach.
从大型单细胞绿藻地中海伞藻（菌株17）的帽状体中制备了纯化的叶绿体组分。从这些叶绿体中获得的高分子量DNA含有至少五个串联排列的10千碱基对（kbp）序列拷贝。这种独特序列存在于所检测生命周期所有阶段的叶绿体DNA中。来自芥菜的叶绿体rDNA克隆与伞藻叶绿体DNA的一些限制性片段杂交，但不与重复序列杂交。将重复序列的一个8-kbp EcoRI-Pst I片段克隆到pBR322中，并用作杂交探针。在所克隆的8-kbp序列与相关物种细圆齿伞藻的叶绿体DNA或菠菜的叶绿体DNA之间未发现同源性。
