Weigle W O
Immunology. 1966 Apr;10(4):377-82.
The ability of rabbits to make a secondary response to certain protein antigens declines either not at all or very slowly with increase in time. The amount of precipitating antibody produced following a second injection of haemocyanin is the same whether the injection is given 2, 7 or 20 months after the primary injection. With BSA, the amount of precipitating antibody produced was the same when the second injection was given either 2 or 7 months after the primary injection, but rabbits given the second injection 20 months after the first produced 40 per cent less precipitating antibody. The level of binding anti-BSA (measured by ammonium sulphate precipitation) produced was not markedly different when the second injection was given, 2, 7 or 20 months after the first injection. Synthesis of antibody persisted in many of the rabbits for as long as 20 months after a single intravenous injection of either haemocyanin or BSA. The antibody to haemocyanin could be detected by precipitation whereas the ammonium sulphate technique had to be used to detect the antibody to BSA.
随着时间的增加，兔子对某些蛋白质抗原产生二次反应的能力要么根本不下降，要么下降得非常缓慢。再次注射血蓝蛋白后产生的沉淀抗体量，无论在初次注射后2个月、7个月还是20个月进行再次注射，都是相同的。对于牛血清白蛋白（BSA），当在初次注射后2个月或7个月进行再次注射时，产生的沉淀抗体量相同，但在初次注射20个月后进行再次注射的兔子产生的沉淀抗体量少40%。当在初次注射后2个月、7个月或20个月进行再次注射时，产生的结合抗牛血清白蛋白（通过硫酸铵沉淀法测量）水平没有明显差异。在单次静脉注射血蓝蛋白或牛血清白蛋白后，许多兔子体内的抗体合成持续长达20个月。血蓝蛋白抗体可通过沉淀法检测，而检测牛血清白蛋白抗体则必须使用硫酸铵技术。
