人绒毛膜生长催乳素重组DNA的构建与分析
Construction and analysis of recombinant DNA for human chorionic somatomammotropin.
作者信息
Shine J, Seeburg P H, Martial J A, Baxter J D, Goodman H M
出版信息
Nature. 1977 Dec 8;270(5637):494-9. doi: 10.1038/270494a0.
DOI:10.1038/270494a0
PMID:593368
Abstract
DNA complementary to mRNA coding for the human polypeptide hormone, chorionic somatomammotropin, has been purified by specific restriction endonuclease digestion and religation before cloning into bacterial plasmids. The primary structure of a major portion of this mRNA species is deduced from the nucleotide sequence of the recombinant DNA.
摘要
编码人类多肽激素绒毛膜生长催乳素的信使核糖核酸（mRNA）的互补脱氧核糖核酸（DNA），在克隆到细菌质粒之前，已通过特定的限制性内切酶消化和重新连接进行了纯化。该信使核糖核酸主要部分的一级结构是从重组DNA的核苷酸序列推导出来的。
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