Jonasson O, Becker E L
J Exp Med. 1966 Mar 1;123(3):509-22. doi: 10.1084/jem.123.3.509.
An antigen-antibody reaction occurring in the perfused sensitized guinea pig lung, has been demonstrated to release kallikrein, a proteolytic enzyme related to the formation of kinins. This lung kallikrein is similar to plasma kallikrein in all properties studied, including susceptibility to the same inhibitors, electrophoretic mobility, and heterogeneity in molecular size. The release of kallikrein during anaphylaxis in the guinea pig lung occurs in the presence of ethylenediaminetetraacetate. Perfusion of ellagic acid into nonsensitized lungs will also release kallikrein, presumably through activation of Hageman factor. On the basis of these findings the hypothesis is suggested that the kallikrein in perfused lung activated by the antigen-antibody reaction is, in fact, plasma kallikrein. It is further suggested that activation of such kallikrein by the antigen-antibody reaction proceeds through Hageman factor.
在灌注的致敏豚鼠肺中发生的抗原-抗体反应，已被证明会释放激肽释放酶，这是一种与激肽形成相关的蛋白水解酶。这种肺激肽释放酶在所有研究的特性上都与血浆激肽释放酶相似，包括对相同抑制剂的敏感性、电泳迁移率以及分子大小的异质性。豚鼠肺过敏反应期间激肽释放酶的释放发生在乙二胺四乙酸存在的情况下。将鞣花酸灌注到未致敏的肺中也会释放激肽释放酶，推测是通过激活哈格曼因子。基于这些发现，提出了一个假说，即由抗原-抗体反应激活的灌注肺中的激肽释放酶实际上是血浆激肽释放酶。进一步表明，抗原-抗体反应对这种激肽释放酶的激活是通过哈格曼因子进行的。
