Eckert E A
J Bacteriol. 1966 May;91(5):1907-10. doi: 10.1128/jb.91.5.1907-1910.1966.
Eckert, Edward A. (University of Michigan, Ann Arbor). Envelope protein(s) derived from influenza virus. J. Bacteriol. 91:1907-1910. 1966.-Lipids were extracted from influenza virus, strain PR8, with methanol-chloroform, and the protein residue was dissolved in 67% glacial acetic acid. Hemagglutinating activity and complement-fixing reactivity were markedly reduced or lost during lipid extraction, and then increased after acetic acid treatment and subsequent dialysis. Evidence is presented that the envelope protein(s) responsible for these activities is dissociated in acetic acid and reassociated at neutral pH.
埃克特,爱德华·A.(密歇根大学,安阿伯分校)。源自流感病毒的包膜蛋白。《细菌学杂志》91:1907 - 1910。1966年。——用甲醇 - 氯仿从PR8株流感病毒中提取脂质,蛋白质残渣溶解于67%的冰醋酸中。在脂质提取过程中,血凝活性和补体结合反应性显著降低或丧失,而在醋酸处理及随后的透析后又有所增加。有证据表明,负责这些活性的包膜蛋白在醋酸中解离,并在中性pH下重新结合。