一种通过免疫酶法提高免疫电泳技术中抗原检测灵敏度的方法。应用于人类甲胎蛋白的定量分析。
An immunoenzymatic method for improving the sensitivity of antigen measurement by electroimmunodiffusion techniques. Application to the quantification of human alpha-fetoprotein.
作者信息
Piñeiro A, Muñoz J R, Perdices A
出版信息
Rev Esp Fisiol. 1976 Jun;32(2):137-42.
A double antibody technique of electroimmunodiffusion, which uses glucose oxidase-labelled sheep antibodies to rabbit immunoglobulins as second antibody, is described. Primary antigen-antibody reaction is carried out with a rabbit antiserum by electroimmundiffusion. The glucose oxidase-labelled immunoreagent, being of general application, can serve for the quantification of different antigens and is here used for measurement of low levels of human alpha-fetoprotein. Reproducible results in the range of 50-800 ng/ml were obtained with a variation coefficient of 5 to 10%.
本文描述了一种双抗体免疫电扩散技术，该技术使用葡萄糖氧化酶标记的羊抗兔免疫球蛋白抗体作为第二抗体。通过免疫电扩散法用兔抗血清进行初次抗原-抗体反应。葡萄糖氧化酶标记的免疫试剂具有广泛的适用性，可用于不同抗原的定量分析，在此用于检测低水平的人甲胎蛋白。在50-800 ng/ml范围内获得了可重复的结果，变异系数为5%至10%。
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