Ikkai T, Ooi T, Noguchi H
Science. 1966 Jun 24;152(3730):1756-7. doi: 10.1126/science.152.3730.1756.
The volume change occurring on polymerizing actin was measured by dilatometry. A large positive value of + 391 ml/mole was obtained for the volume change during the transformation of G- to F-actin. This large increase in volume could be interpreted as arising from the local change in the ordered water structure on the protein's surface at polymerizing sites.
通过膨胀测量法测定了肌动蛋白聚合时发生的体积变化。在G-肌动蛋白向F-肌动蛋白转变过程中,体积变化获得了+391毫升/摩尔的大正值。这种体积的大幅增加可以解释为聚合位点处蛋白质表面有序水结构的局部变化所致。