来自植物组织的腺苷酸琥珀酸合成酶和腺苷酸琥珀酸裂解酶。
Adenylosuccinate synthetase and adenylosuccinate lyase from plant tissues.
作者信息
Hatch M D
出版信息
Biochem J. 1966 Jan;98(1):198-203. doi: 10.1042/bj0980198.
Abstract
- Enzymes that convert IMP into adenylosuccinate (adenylosuccinate synthetase) and adenylosuccinate into AMP (adenylosuccinate lyase) were isolated from wheat germ and pea seeds and their properties are described. 2. These enzymes were purified approx. 200-fold from wheat-germ extracts. 3. A heat treatment provided adenylosuccinate lyase free of adenylosuccinate synthetase but the behaviour of the two enzymes was almost identical in a number of fractionation procedures. The two activities were finally separated by filtration on Sephadex G-100. 4. The identification of these enzymes in plant tissues is discussed in relation to the pathway of purine synthesis.
摘要
- 从小麦胚芽和豌豆种子中分离出将肌苷酸（IMP）转化为腺苷酸琥珀酸（腺苷酸琥珀酸合成酶）以及将腺苷酸琥珀酸转化为AMP（腺苷酸琥珀酸裂解酶）的酶，并对其性质进行了描述。2. 这些酶从麦胚提取物中纯化了约200倍。3. 热处理得到了不含腺苷酸琥珀酸合成酶的腺苷酸琥珀酸裂解酶，但在一些分级分离程序中，这两种酶的行为几乎相同。最终通过在葡聚糖G - 100上过滤将这两种活性分开。4. 结合嘌呤合成途径讨论了这些酶在植物组织中的鉴定。
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