在存在高分子量底物的情况下对酶进行葡聚糖凝胶过滤。
Dextran-gel filtration of enzymes in the presence of their high-molecular-weight substrates.
作者信息
Auricchio F, Bruni C B
出版信息
Biochem J. 1966 Jan;98(1):290-2. doi: 10.1042/bj0980290.
Abstract
- Trypsin and ribonuclease were filtered through dextran gel (Sephadex G-100) columns in the absence and presence of their respective substrates. In the presence of their high-molecular-weight substrates the enzymes emerged earlier from the columns. This appeared to be due to the reversible formation of specific enzyme-substrate complexes. 2. The possibility of separation of an enzyme from other proteins with similar molecular weights was demonstrated with trypsin and cytochrome c in the presence of casein.
摘要
- 在有无各自底物的情况下，将胰蛋白酶和核糖核酸酶通过葡聚糖凝胶（葡聚糖G - 100）柱进行过滤。在存在其高分子量底物时，酶从柱中洗脱得更早。这似乎是由于特异性酶 - 底物复合物的可逆形成。2. 在酪蛋白存在的情况下，用胰蛋白酶和细胞色素c证明了将一种酶与其他分子量相似的蛋白质分离的可能性。
相似文献
1
Dextran-gel filtration of enzymes in the presence of their high-molecular-weight substrates.在存在高分子量底物的情况下对酶进行葡聚糖凝胶过滤。
Biochem J. 1966 Jan;98(1):290-2. doi: 10.1042/bj0980290.
2
Estimation of the molecular weights of proteins by Sephadex gel-filtration.用葡聚糖凝胶过滤法估算蛋白质的分子量
Biochem J. 1964 May;91(2):222-33. doi: 10.1042/bj0910222.
3
GEL FILTRATION APPLIED TO THE STUDY OF LIPASES AND OTHER ESTERASES.凝胶过滤法在脂肪酶及其他酯酶研究中的应用
Biochem J. 1965 Mar;94(3):642-50. doi: 10.1042/bj0940642.
4
Estimation of molecular parameters of proteins by gel chromatography on Sephadex G-150.通过Sephadex G - 150凝胶色谱法估算蛋白质的分子参数
Anal Biochem. 1970 Nov;38(1):285-8. doi: 10.1016/0003-2697(70)90179-x.
5
[Physical bases of the method of determining the molecular sizes of proteins using gel filtration].[利用凝胶过滤法测定蛋白质分子大小的方法的物理基础]
Biokhimiia. 1970 May-Jun;35(3):562-4.
6
Gel filtration of modified bovine serum on Sephadex G-200.经修饰的牛血清在葡聚糖凝胶G - 200上的凝胶过滤。
J Chromatogr. 1965 Jul;19(1):64-7. doi: 10.1016/s0021-9673(01)99426-9.
7
Ribonuclease activity in commercial crystalline trypsin and a method for removal.商业结晶胰蛋白酶中的核糖核酸酶活性及去除方法。
Science. 1968 Nov 22;162(3856):912-3. doi: 10.1126/science.162.3856.912.
8
Serum trypsin inhibitor: a comparison of assay methods using amide and casein substrates.血清胰蛋白酶抑制剂：使用酰胺和酪蛋白底物的检测方法比较
Enzymol Biol Clin (Basel). 1968;9(1):1-9. doi: 10.1159/000458229.
9
[Characteristics of extracellular proteolytic enzymes of Actinomyces fradiae 0072].
Prikl Biokhim Mikrobiol. 1976 Jul-Aug;12(4):563-71.
10
A RELATIONSHIP BETWEEN THE MOLECULAR WEIGHTS OF MACROMOLECULES AND THEIR ELUTION VOLUMES BASED ON A MODEL FOR SEPHADEX GEL FILTRATION.基于葡聚糖凝胶过滤模型的大分子分子量与其洗脱体积之间的关系。
Arch Biochem Biophys. 1964 Sep;107:471-8. doi: 10.1016/0003-9861(64)90303-0.
引用本文的文献
1
Purification of an acid alpha-glucosidase by dextran-gel filtration.通过葡聚糖凝胶过滤法纯化酸性α-葡萄糖苷酶
Biochem J. 1967 Oct;105(1):35-8. doi: 10.1042/bj1050035.
2
Inactivation of tyrosine aminotransferase in neutral homogenates and rat liver slices.中性匀浆和大鼠肝切片中酪氨酸转氨酶的失活
Biochem J. 1972 Oct;129(5):1131-8. doi: 10.1042/bj1291131.
本文引用的文献
1
A spectrophotometric determination of trypsin and chymotrypsin.胰蛋白酶和胰凝乳蛋白酶的分光光度测定法
Biochim Biophys Acta. 1955 Apr;16(4):570-5. doi: 10.1016/0006-3002(55)90280-8.
2
[THE DETERMINATION OF THE MOLECULAR WEIGHT OF ENZYMES BY DEXTRAN GEL FILTRATION].[用葡聚糖凝胶过滤法测定酶的分子量]
Biochem Z. 1964 Aug 11;340:321-5.
3
4
Estimation of molecular weights of proteins by gel filtration.通过凝胶过滤法估算蛋白质的分子量
Nature. 1962 Oct 6;196:36-9. doi: 10.1038/196036a0.
5
[Relative determination of the molecular weight of unurified enzymes by the molecular sieve method].[用分子筛法相对测定未纯化酶的分子量]
C R Hebd Seances Acad Sci. 1963 Jun 10;256:5224-7.
6
7
Physicochemical properties of pig and horse heart mitochondrial malate dehydrogenase.猪和马心脏线粒体苹果酸脱氢酶的物理化学性质
J Biol Chem. 1963 May;238:1861-8.
8
Peptides obtained by tryptic hydrolysis of performic acid-oxidized ribonuclease.通过对过甲酸氧化的核糖核酸酶进行胰蛋白酶水解得到的肽段。
J Biol Chem. 1956 Apr;219(2):623-42.
9
Studies on the gross structure, cross-linkages, and terminal sequences in ribonuclease.关于核糖核酸酶的总体结构、交联和末端序列的研究。
J Biol Chem. 1954 Mar;207(1):201-10.
10
Estimation of the molecular weights of proteins by Sephadex gel-filtration.用葡聚糖凝胶过滤法估算蛋白质的分子量
Biochem J. 1964 May;91(2):222-33. doi: 10.1042/bj0910222.
Zotero 插件