Tunis M
Science. 1968 Nov 22;162(3856):912-3. doi: 10.1126/science.162.3856.912.
Several preparations of crystalline trypsin hydrolyze RNA because of contaminating ribonuclease activity. Filtration of these materials through Sephadex G-50 yields a trypsin devoid of ribonuclease activity and having a proteolytic specific activity about 70 percent of the starting material.
由于存在核糖核酸酶活性的污染,几种结晶胰蛋白酶制剂会水解RNA。将这些物质通过葡聚糖G - 50过滤,可得到一种不含核糖核酸酶活性且蛋白水解比活性约为起始材料70%的胰蛋白酶。