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通过与互补DNA的杂交分析确定黄瓜花叶病毒四种RNA种类之间的序列同源性。

Determination of the sequence homology between the four RNA species of cucumber mosaic virus by hybridization analysis with complementary DNA.

作者信息

Gould A R, Symons R H

出版信息

Nucleic Acids Res. 1977 Nov;4(11):3787-802. doi: 10.1093/nar/4.11.3787.

DOI:10.1093/nar/4.11.3787
PMID:593886
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC343200/
Abstract

The method of Taylor etal., (11) has been used to transcribe complementary DNA probes from the four major RNA species of cucumber mosaic virus (RNAs 1 - 4 in order of decreasing molecular weight). Analysis of the kinetics of hybridization of these probes in homologous and heterologous complementary DNA-RNA hybridization reactions has shown that the sequence of the smallest RNA (RNA 4), which contains the coat protein gene, is present within RNA 3. RNAs 1 and 2 are unique RNA molecules while each has a region of approximately 300 nucleotides in common with RNA 4.Images

摘要

泰勒等人（11）的方法已被用于从黄瓜花叶病毒的四种主要RNA种类（按分子量递减顺序为RNA 1 - 4）转录互补DNA探针。对这些探针在同源和异源互补DNA - RNA杂交反应中的杂交动力学分析表明，包含外壳蛋白基因的最小RNA（RNA 4）的序列存在于RNA 3中。RNA 1和RNA 2是独特的RNA分子，而它们各自都有一个与RNA 4共有的约300个核苷酸的区域。图片

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c003/343200/6c2f4dd82c7d/nar00484-0117-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c003/343200/6c2f4dd82c7d/nar00484-0117-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c003/343200/6c2f4dd82c7d/nar00484-0117-a.jpg

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本文引用的文献

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The loss of ribosomal ribonucleic acid during the preparationof nucleic acid from certain plant tissues by the detergent-phenol method.在通过去污剂-苯酚法从某些植物组织制备核酸的过程中核糖体核糖核酸的损失。
Biochem J. 1968 Dec;110(3):605-6. doi: 10.1042/bj1100605.
2
Evidence for three functional RNA species in several strains of cucumber mosaic virus.几种黄瓜花叶病毒株系中三种功能性RNA种类的证据。
J Gen Virol. 1974 Jan;22(1):81-93. doi: 10.1099/0022-1317-22-1-81.
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Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae.
Arch Virol. 1987;96(3-4):225-40. doi: 10.1007/BF01320962.
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The use of recombinant DNA probes to group and type orbiviruses. A comparison of Australian and South African isolates.使用重组DNA探针进行环状病毒的分组和分型。澳大利亚和南非分离株的比较。
Arch Virol. 1988;99(3-4):205-20. doi: 10.1007/BF01311070.
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Transcription of tomato ribosomal DNA and the organization of the intergenic spacer.番茄核糖体DNA的转录及基因间隔区的组织
Mol Gen Genet. 1990 Mar;221(1):103-12. doi: 10.1007/BF00280374.
6
A study of barley stripe mosaic virus (BSMV) genome. I. Determination of sequence homology between BSMV RNA species.大麦条纹花叶病毒（BSMV）基因组研究。I. BSMV RNA种类间序列同源性的测定。
Mol Gen Genet. 1979 Aug;175(1):89-92. doi: 10.1007/BF00267859.
7
Extensive sequence homology at the 3'-termini of the four RNAs of cucumber mosaic virus.黄瓜花叶病毒四种RNA 3'末端的广泛序列同源性。
Nucleic Acids Res. 1979 Oct 25;7(4):825-37. doi: 10.1093/nar/7.4.825.
8
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Nucleic Acids Res. 1978 Nov;5(11):4423-30. doi: 10.1093/nar/5.11.4423.
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