以小牛胸腺DNA水解物为引物的芜菁黄花叶病毒RNA逆转录：纯化的cDNA产物的特性分析
Reverse transcription of turnip yellow mosaic virus RNA primed with calf-thymus DNA hydrolysate: characterization of the purified cDNA product.
作者信息
Kummert J, Kettmann R
出版信息
Nucleic Acids Res. 1978 Nov;5(11):4423-30. doi: 10.1093/nar/5.11.4423.
Abstract
Complementary DNA was transcribed from turnip yellow mosaic virus RNA, using the method of Taylor et al. (1). The purified cDNA thus obtained sedimented between 2 and 4 S and was a mostly uniform transcript of template RNA. It hybridized with a sharp transition to homologous TYMV-RNA (Crt 1/2 = 2.7 x 10(-2)), but showed a low level of hybridization (less than 5%) to the RNAs of two other tymoviruses, namely Andean potato latent virus and eggplant mosaic virus.
摘要
采用泰勒等人（1）的方法，从芜菁黄花叶病毒RNA转录互补DNA。由此获得的纯化互补DNA在2至4S之间沉降，并且是模板RNA的大致均匀转录本。它与同源芜菁黄花叶病毒RNA发生急剧转变的杂交（Crt 1/2 = 2.7×10⁻²），但与另外两种芜菁黄花叶病毒，即安第斯马铃薯潜隐病毒和茄子花叶病毒的RNA杂交水平较低（低于5%）。
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Reverse transcription of turnip yellow mosaic virus RNA primed with calf-thymus DNA hydrolysate: characterization of the purified cDNA product.以小牛胸腺DNA水解物为引物的芜菁黄花叶病毒RNA逆转录：纯化的cDNA产物的特性分析
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本文引用的文献
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