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以小牛胸腺DNA水解物为引物的芜菁黄花叶病毒RNA逆转录:纯化的cDNA产物的特性分析

Reverse transcription of turnip yellow mosaic virus RNA primed with calf-thymus DNA hydrolysate: characterization of the purified cDNA product.

作者信息

Kummert J, Kettmann R

出版信息

Nucleic Acids Res. 1978 Nov;5(11):4423-30. doi: 10.1093/nar/5.11.4423.

DOI:10.1093/nar/5.11.4423
PMID:82938
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342759/
Abstract

Complementary DNA was transcribed from turnip yellow mosaic virus RNA, using the method of Taylor et al. (1). The purified cDNA thus obtained sedimented between 2 and 4 S and was a mostly uniform transcript of template RNA. It hybridized with a sharp transition to homologous TYMV-RNA (Crt 1/2 = 2.7 x 10(-2)), but showed a low level of hybridization (less than 5%) to the RNAs of two other tymoviruses, namely Andean potato latent virus and eggplant mosaic virus.

摘要

采用泰勒等人(1)的方法,从芜菁黄花叶病毒RNA转录互补DNA。由此获得的纯化互补DNA在2至4S之间沉降,并且是模板RNA的大致均匀转录本。它与同源芜菁黄花叶病毒RNA发生急剧转变的杂交(Crt 1/2 = 2.7×10⁻²),但与另外两种芜菁黄花叶病毒,即安第斯马铃薯潜隐病毒和茄子花叶病毒的RNA杂交水平较低(低于5%)。

相似文献

1
Reverse transcription of turnip yellow mosaic virus RNA primed with calf-thymus DNA hydrolysate: characterization of the purified cDNA product.以小牛胸腺DNA水解物为引物的芜菁黄花叶病毒RNA逆转录:纯化的cDNA产物的特性分析
Nucleic Acids Res. 1978 Nov;5(11):4423-30. doi: 10.1093/nar/5.11.4423.
2
Properties of the DNA product of reverse transcription of turnip yellow mosaic virus RNA preparations.芜菁黄花叶病毒RNA制剂逆转录产生的DNA产物的特性
Virology. 1977 Sep;81(2):449-54. doi: 10.1016/0042-6822(77)90159-3.
3
Synthesis of Sindbis virus complementary DNA by avian myeloblastosis virus RNA-directed DNA polymerase.禽成髓细胞瘤病毒RNA指导的DNA聚合酶合成辛德毕斯病毒互补DNA。
Biochim Biophys Acta. 1978 Jul 24;519(2):348-55. doi: 10.1016/0005-2787(78)90087-4.
4
Efficient reverse transcription of cowpea mosaic virus RNAs.豇豆花叶病毒RNA的高效逆转录
Nucleic Acids Res. 1978 Dec;5(12):4643-61. doi: 10.1093/nar/5.12.4643.
5
RNA primer used in synthesis of anticomplementary DNA by reverse transcriptase of avian myeloblastosis virus.禽成髓细胞瘤病毒逆转录酶用于合成反互补DNA时所使用的RNA引物。
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1316-20. doi: 10.1073/pnas.77.3.1316.
6
Reverse transcription using the genomic RNA of two tymoviruses as template, and calf thymus DNA hydrolysate as primer [proceedings].以两种番茄病毒的基因组RNA为模板,小牛胸腺DNA水解物为引物进行反转录[会议论文集]
Arch Int Physiol Biochim. 1978 Oct;86(4):917-8.
7
Synthesis in high yield of complementary DNA of retroviral RNA.逆转录病毒RNA互补DNA的高产合成。
Prep Biochem. 1980;10(4):483-93. doi: 10.1080/00327488008061745.
8
Exogenous primer-independent cDNA synthesis with commercial reverse transcriptase preparations on plant virus RNA templates.使用商业逆转录酶制剂在植物病毒RNA模板上进行不依赖外源性引物的cDNA合成。
Anal Biochem. 1992 May 15;203(1):163-5. doi: 10.1016/0003-2697(92)90058-f.
9
The classification of tymoviruses by cDNA-RNA hybridization and other measures of relatedness.通过cDNA-RNA杂交及其他相关性检测手段对番茄丛矮病毒属进行分类。
Arch Virol. 1987;96(3-4):225-40. doi: 10.1007/BF01320962.
10
Gene specific priming of complementary DNA synthesis.基因特异性引发互补DNA合成。
Mol Biol Rep. 1976 Nov;3(2):167-73. doi: 10.1007/BF00423231.

本文引用的文献

1
Properties of nucleoprotein fractions isolated from turnip yellow mosaic virus preparations.从芜菁黄花叶病毒制剂中分离出的核蛋白组分的特性。
Virology. 1960 Dec;12:521-39. doi: 10.1016/0042-6822(60)90176-8.
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In vitro pulse-labeling of the replicative forms of bromegrass mosaic virus RNA.雀麦花叶病毒RNA复制形式的体外脉冲标记
Virology. 1974 Feb;57(2):314-20. doi: 10.1016/0042-6822(74)90171-8.
3
Possible heterogeneity of 5'-terminal of turnip yellow mosaic virus RNA.芜菁黄花叶病毒RNA 5′末端可能存在的异质性。
Virology. 1973 Apr;52(2):578-80. doi: 10.1016/0042-6822(73)90354-1.
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Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae.米曲霉单链特异性核酸酶的纯化及其他特性
Eur J Biochem. 1973 Feb 15;33(1):192-200. doi: 10.1111/j.1432-1033.1973.tb02669.x.
5
Differences between the integration of avian myeloblastosis virus DNA in leukemic cells and of endogenous viral DNA in normal chicken cells.禽成髓细胞瘤病毒DNA在白血病细胞中的整合与正常鸡细胞中内源性病毒DNA整合之间的差异。
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3152-6. doi: 10.1073/pnas.71.8.3152.
6
A study of nucleotide sequence homology between strains of tobacco mosaic virus.烟草花叶病毒株系间核苷酸序列同源性的研究。
Virology. 1976 Sep;73(2):413-8. doi: 10.1016/0042-6822(76)90402-5.
7
Lack of molecular hybridization between RNAs of two strains of TMV: a reconsideration of the criteria for strain relationships.两种烟草花叶病毒株系的RNA之间缺乏分子杂交:对株系关系标准的重新思考。
Virology. 1977 Oct 1;82(1):237-41. doi: 10.1016/0042-6822(77)90047-2.
8
Characterization of a satellite RNA associated with cucumber mosaic virus.与黄瓜花叶病毒相关的一种卫星RNA的特性分析
Virology. 1978 Feb;84(2):443-55. doi: 10.1016/0042-6822(78)90261-1.
9
Determination of the sequence homology between the four RNA species of cucumber mosaic virus by hybridization analysis with complementary DNA.通过与互补DNA的杂交分析确定黄瓜花叶病毒四种RNA种类之间的序列同源性。
Nucleic Acids Res. 1977 Nov;4(11):3787-802. doi: 10.1093/nar/4.11.3787.
10
Isolation of the mouse mammary tumor virus sequences not transmitted as germinal provirus in the C3H and RIII mouse strains.在C3H和RIII小鼠品系中未作为生殖系前病毒传播的小鼠乳腺肿瘤病毒序列的分离。
J Virol. 1977 Mar;21(3):986-95. doi: 10.1128/JVI.21.3.986-995.1977.