染色质颗粒之间的亚基关联。

Subunit associations among chromatin particles.

作者信息

Campbell A M, Cotter R I

出版信息

Nucleic Acids Res. 1977 Nov;4(11):3877-86. doi: 10.1093/nar/4.11.3877.

DOI:10.1093/nar/4.11.3877

PMID:593892

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC343207/

Abstract

The self-association of oligonucleosomal chromatin particles in solution has been studied by light scattering and sedimentation. In the absence of magnesium ions no association is observed. In the presence of 70mM sodium or 2mM magnesium ions mono, di, tri and tetranucleosomes self-associate only if they contain bound histone 1. This association leads to the formation of compact aggregates and is continuous and non-cooperative. The relevance to higher order arrangements of nucleosomes is discussed.

摘要

通过光散射和沉降法研究了溶液中寡核小体染色质颗粒的自缔合现象。在没有镁离子的情况下，未观察到缔合现象。在存在70mM钠或2mM镁离子的情况下，单核小体、双核小体、三核小体和四核小体只有在含有结合的组蛋白1时才会自缔合。这种缔合导致形成紧密的聚集体，并且是连续的且非协同的。文中讨论了其与核小体更高层次排列的相关性。