狂犬病病毒的密度梯度离心研究。
Density gradient centrifugation studies on rabies virus.
作者信息
Neurath A R, Wiktor T J, Koprowski H
出版信息
J Bacteriol. 1966 Jul;92(1):102-6. doi: 10.1128/jb.92.1.102-106.1966.
Abstract
Neurath, A. Robert (The Wistar Institute, Philadelphia, Pa.), Tadeusz J. Wiktor, and Hilary Koprowski. Density gradient centrifugation studies on rabies virus. J. Bacteriol. 92:102-106. 1966.-Cesium chloride density gradient centrifugation of rabies virus revealed a heterogeneous population of infectious virus particles, the majority of which showed a density of 1.20 g/ml. From results obtained by rate zonal centrifugation in preformed sucrose gradients, it was possible to calculate a sedimentation coefficient of about 600S for rabies virus. Sedimentation coefficients of about 23S and 10S were calculated for two soluble rabies antigens present in infected tissue-culture fluids, and they showed a density of 1.26 g/ml in cesium chloride solutions.
摘要
诺伊拉特，A. 罗伯特（宾夕法尼亚州费城威斯塔研究所），塔德乌什·J. 维克托，以及希拉里·科普罗夫斯基。狂犬病病毒的密度梯度离心研究。《细菌学杂志》92:102 - 106。1966年。——狂犬病病毒的氯化铯密度梯度离心显示出一群异质性的感染性病毒颗粒，其中大多数的密度为1.20克/毫升。根据在预制蔗糖梯度中进行速率区带离心所获得的结果，有可能计算出狂犬病病毒的沉降系数约为600S。对于感染的组织培养液中存在的两种可溶性狂犬病抗原，计算出的沉降系数约为23S和10S，并且它们在氯化铯溶液中的密度为1.26克/毫升。
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