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关于纤细裸藻合成植物硫脂的一些观察。

Some observations on the biosynthesis of the plant sulpholipid by Euglena gracilis.

作者信息

Davies W H, Mercer E I, Goodwin T W

出版信息

Biochem J. 1966 Feb;98(2):369-73. doi: 10.1042/bj0980369.

Abstract
  1. dl-Cysteine decreases the uptake of (35)SO(4) (2-) by Euglena gracilis but does not decrease the relative incorporation of the isotope into sulpholipid; cysteic acid, on the other hand, does not affect the uptake of (35)SO(4) (2-) but does dilute out its incorporation into the sulpholipid. 2. Both l-[(35)S]cysteic acid and dl-+meso-[3-(14)C]cysteic acid appear almost exclusively in 6-sulphoquinovose. 3. Molybdate inhibits the incorporation of (35)SO(4) (2-) into sulpholipid but not its uptake into the cells; this suggests that adenosine 3'-phosphate 5'-sulphatophosphate may be concerned with the biosynthesis of sulpholipid, and it was shown to be formed by chloroplast fragments. 4. An outline scheme for sulpholipid biosynthesis based on these observations is discussed.
摘要
  1. 消旋半胱氨酸可降低纤细裸藻对(35)SO(4)(2-)的摄取,但不会降低该同位素在硫脂中的相对掺入量;另一方面,半胱氨酸不影响(35)SO(4)(2-)的摄取,但会稀释其在硫脂中的掺入。2. L-[(35)S]半胱氨酸和消旋+内消旋-[3-(14)C]半胱氨酸几乎都只出现在6-磺基奎诺糖中。3. 钼酸盐可抑制(35)SO(4)(2-)掺入硫脂,但不影响其进入细胞;这表明3'-磷酸腺苷5'-硫酸磷酸可能与硫脂的生物合成有关,并且已证明它是由叶绿体片段形成的。4. 基于这些观察结果讨论了硫脂生物合成的概述方案。

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Photophosphorylation by isolated chloroplasts of Euglena gracilis.纤细裸藻分离叶绿体的光合磷酸化作用。
Biochem Biophys Res Commun. 1966 Aug 12;24(3):329-33. doi: 10.1016/0006-291x(66)90159-8.

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