White R H
J Bacteriol. 1984 Jul;159(1):42-6. doi: 10.1128/jb.159.1.42-46.1984.
The biosynthesis of the sulfonolipid 2-amino-3-hydroxy-15-methylhexadecane-1-sulfonic acid (capnine) was studied by measuring the incorporation of possible precursors into the lipid by cells grown in the presence of precursors which were labeled with stable isotopes. Cells grown on yeast extract in the presence of DL-[3,3-2H2]serine contained 40.1 mol% of the protein-bound serine and 5.0 mol% of the protein-bound cysteine derived from the labeled serine. Cells grown in the presence of DL-[3,3-2H2]cystine acid contained 86.4 mol% of the molecules that had two deuteriums. These results are consistent with the possibility that biosynthesis of capnine occurs by the condensation of 13-methylmyristoyl-coenzyme A with cysteic acid, in a reaction analogous to the condensation of a palmitoyl-coenzyme A with serine to form 3-keto-sphinganine during the biosynthesis of sphingolipids.
通过测量在含有用稳定同位素标记的前体的情况下生长的细胞将可能的前体掺入脂质中的情况，对磺脂2-氨基-3-羟基-15-甲基十六烷-1-磺酸（癸磺胆胺）的生物合成进行了研究。在DL-[3,3-²H₂]丝氨酸存在下于酵母提取物上生长的细胞含有40.1摩尔%的与蛋白质结合的源自标记丝氨酸的丝氨酸和5.0摩尔%的与蛋白质结合的半胱氨酸。在DL-[3,3-²H₂]胱氨酸存在下生长的细胞含有86.4摩尔%的具有两个氘的分子。这些结果与癸磺胆胺生物合成可能通过13-甲基肉豆蔻酰辅酶A与半胱氨酸的缩合发生的可能性一致，该反应类似于在鞘脂生物合成过程中棕榈酰辅酶A与丝氨酸缩合形成3-酮鞘氨醇的反应。
