沼泽红假单胞菌血红素色素的电泳及其他研究：细胞色素552和类细胞色素c
Electrophoretic and other studies on haem pigments from Rhodopseudomonas palustris: cytochrome 552 and cytochromoid c.
作者信息
Henderson R W, Nankiville D D
出版信息
Biochem J. 1966 Feb;98(2):587-93. doi: 10.1042/bj0980587.
Abstract
- Cytochrome 552 and cytochromoid c were extracted from Rhodopseudomonas palustris cells, purified and obtained in crystalline form. 2. Extinction ratios and amino acid compositions of the two pigments are reported. 3. When subjected to starch-gel electrophoresis in borate buffer, pH8.8, each pigment migrated towards the cathode; oxidized cytochromoid c migrated more rapidly than its reduced form. 4. By a determination of electrophoretic mobilities in buffers of I0.1 by using the moving-boundary method, the isoelectric point of cytochrome 552 was found to be at pH10.6 and that of cytochromoid c at pH9.7. 5. As obtained, cytochrome 552 was non-autoxidizable; cytochromoid c was autoxidizable but became considerably less so on alkaline treatment. 6. Discussion of the results includes a consideration of the isoelectric points of the pigments in terms of their amino acid composition.
摘要
- 从沼泽红假单胞菌细胞中提取细胞色素552和类细胞色素c，进行纯化并获得结晶形式。2. 报告了这两种色素的消光比和氨基酸组成。3. 在pH8.8的硼酸盐缓冲液中进行淀粉凝胶电泳时，每种色素都向阴极迁移；氧化型类细胞色素c比其还原型迁移得更快。4. 通过使用移动边界法测定在离子强度为0.1的缓冲液中的电泳迁移率，发现细胞色素552的等电点在pH10.6，类细胞色素c的等电点在pH9.7。5. 所获得的细胞色素552不可自氧化；类细胞色素c可自氧化，但经碱性处理后自氧化能力大大降低。6. 对结果的讨论包括根据色素的氨基酸组成对等电点的考虑。
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