单宁酸 - 戊二醛在弹性纤维及与弹性相关纤维研究中的应用
The use of tannic acid-glutaraldehyde in the study of elastic and elastic-related fibers.
作者信息
Cotta-Pereira G, Rodrigo F G, David-Ferreira J F
出版信息
Stain Technol. 1976 Jan;51(1):7-11. doi: 10.3109/10520297609116662.
PMID:59416
Abstract
Fixation with tannic acid-glutaraldehyde permits distinction of oxytalan, elaunin and elastic fibers in the electron microscope. The results obtained using tannic acid at concentrations of 1.0%, 0.5% and 0.25% in 3% glutaraldehyde were compared. The 0.25% concentration is recommended for studying fine details of connective fibrils and for regular staining of elastin.
摘要
用鞣酸 - 戊二醛固定可在电子显微镜下区分耐酸纤维、弹力素和弹性纤维。比较了在3%戊二醛中使用浓度为1.0%、0.5%和0.25%的鞣酸所获得的结果。推荐使用0.25%的浓度来研究结缔组织纤维的细微细节以及弹性蛋白的常规染色。
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