Cotta-Pereira G, Rodrigo F G, David-Ferreira J F
Adv Exp Med Biol. 1977;79:19-30. doi: 10.1007/978-1-4684-9093-0_3.
Using the tannic acid-glutaraldehyde fixation it was possible to clearly visualize, with reproducible results, the elastic fiber amorphous material because of its density. The microfibrillar component was also evidenced with a good performance. With this technique the ultrastructural patterns of the oxytalan and elaunin fibers were also demonstrated. The oxytalan fibers appeared as a bundle of microfibrils similar to elastic microfibrils without amorphous material. The elaunin fibers presented a dispersed amorphous material intermingled among the microfibrils. It was suggested by ultrastructural evidences that oxytalan and elaunin fibers may represent interruption in successive phases in the development of the elastic fibers.
使用单宁酸 - 戊二醛固定法，由于弹性纤维无定形物质的密度，能够清晰地观察到其结构，且结果具有可重复性。微原纤维成分也得到了良好的显示。通过这种技术，还展示了氧化弹性纤维和弹力素纤维的超微结构模式。氧化弹性纤维呈现为一束类似于弹性微原纤维的微原纤维，没有无定形物质。弹力素纤维则呈现出分散在微原纤维之间的无定形物质。超微结构证据表明，氧化弹性纤维和弹力素纤维可能代表弹性纤维发育连续阶段中的中断。
