Warren B, Johnson A G, Hoobler S W
J Exp Med. 1966 Jun 1;123(6):1109-28. doi: 10.1084/jem.123.6.1109.
Rabbit antibody capable of neutralizing the pressor activity of the enzyme renin, derived from hog kidneys, was characterized with respect to type and capacity to react serologically. Three antirenin antibodies were detected, both a precipitating and nonprecipitating, neutralizing antibody as well as a neutralizing antibody capable of being absorbed by inactivated renin. The precipitating, neutralizing antibody was purified and used successfully in an assay for hog renin by means of a hemagglutination inhibition test. By immunofluorescent methods utilizing the purified antiserum, renin was found to be localized in the macula densa of the distal convoluted tubules and the juxtaglomerular cells of the afferent arteriole in both hog and dog kidneys.
能够中和源自猪肾的肾素酶升压活性的兔抗体，在血清学反应类型和能力方面进行了表征。检测到三种抗肾素抗体，一种是沉淀性和非沉淀性的中和抗体，以及一种能被灭活肾素吸收的中和抗体。纯化了沉淀性中和抗体，并通过血凝抑制试验成功地用于猪肾素的测定。利用纯化的抗血清通过免疫荧光法发现，在猪和狗的肾脏中，肾素定位于远曲小管的致密斑和入球小动脉的球旁细胞中。
