Wassersug R J
Stain Technol. 1976 Mar;51(2):131-4. doi: 10.3109/10520297609116684.
This paper describes a modification of the Simons and Van Horn (1971) procedure for rendering cartilage blue, bone red, and soft tissue translucent or transparent in whole vertebrate specimens. Alcian blue and alizarin red S are used to stain cartilage and bone respectively. In our procedure formalin is used as a fixative. This is a significant modification because formalin is the common fixative for museum specimens. This clearing and staining procedure is thus readily applicable to comparative studies in anatomy, embryology and systematic zoology.
本文描述了对西蒙斯和范霍恩（1971年）方法的一种改进，该方法可使整个脊椎动物标本中的软骨呈蓝色、骨骼呈红色，软组织呈半透明或透明状。阿尔辛蓝和茜素红S分别用于染色软骨和骨骼。在我们的方法中，福尔马林用作固定剂。这是一个重大的改进，因为福尔马林是博物馆标本常用的固定剂。因此，这种透明和染色方法很容易应用于解剖学、胚胎学和系统动物学的比较研究。
