Turianskyj F H, Gyenes L
Transplantation. 1976 Jul;22(1):24-30. doi: 10.1097/00007890-197607000-00004.
The effect of neuraminidase treatment on the antigenicity of sarcoma I (Sal) and mastocytoma P-815-X2 cells was compared, both in terms of their reaction with various antisera (C57BL/6 anti-SaI, C57BL/6 anti-mastocytoma, rabbit and horse ALS) and complements (guinea pig, rabbit) or with sensitized lymphocytes (C57BL/6 anti-SaI, C57BL/6 anti-mastocytoma, A anti-mastocytoma). With sensitized lymphocytes the reactivity of neuraminidase-treated tumor cells was similar to that of nontreated cells. In contrast, after neuraminidase treatment, the tumor cells showed increased specific lysis with the antisera and complement. Rabbit complement was highly cytotoxic to the neuraminidase-treated cells but this nonspecific activity could be removed by absorption with agar or agarose. The results of this study would strongly suggest that neuraminidase treatment does not change the antigenic determinants of tumor cells but increases their interaction with complement after removal of negatively charged sialic acids.
比较了神经氨酸酶处理对肉瘤I（Sal）和肥大细胞瘤P - 815 - X2细胞抗原性的影响，这是通过它们与各种抗血清（C57BL / 6抗Sal、C57BL / 6抗肥大细胞瘤、兔和马抗淋巴细胞血清）和补体（豚鼠、兔）的反应，或者与致敏淋巴细胞（C57BL / 6抗Sal、C57BL / 6抗肥大细胞瘤、A抗肥大细胞瘤）的反应来进行的。对于致敏淋巴细胞，经神经氨酸酶处理的肿瘤细胞的反应性与未处理的细胞相似。相比之下，经神经氨酸酶处理后，肿瘤细胞与抗血清和补体的特异性裂解增加。兔补体对经神经氨酸酶处理的细胞具有高度细胞毒性，但这种非特异性活性可以通过用琼脂或琼脂糖吸收来去除。这项研究的结果强烈表明，神经氨酸酶处理不会改变肿瘤细胞的抗原决定簇，但在去除带负电荷的唾液酸后会增加它们与补体的相互作用。
