Notkins A L, Mahar S, Scheele C, Goffman J
J Exp Med. 1966 Jul 1;124(1):81-97. doi: 10.1084/jem.124.1.81.
If viremic sera from mice chronically infected with lactic dehydrogenase virus (LDV) were first treated with ether or ultraviolet light to inactivate the infectious virus, neutralizing antibody could be demonstrated. Significant amounts of antibody, however, were not detected until the mice had been infected for about 2(1/2) months and its presence did not result in the elimination of the chronic viremia. Virus isolated from sera containing neutralizing antibody was found to be relatively resistant to neutralization by anti-LDV. Further studies revealed that the resistant virus existed in the form of an infectious virus-antibody complex (sensitized virus). The presence of such a complex was demonstrated by the fact that the virus fraction which persisted after in vivo or in vitro exposure to mouse anti-LDV was readily neutralized by goat anti-mouse sera or goat anti-mouse gamma-globulin, whereas virus that had not been previously exposed to mouse anti-LDV was completely resistant to neutralization by goat anti-mouse sera. These findings suggest that (a) sensitization may play an important role in the resistance and susceptibility of a virus to neutralization by antiviral antibody, and (b) an anti-gamma-globulin may prove useful in neutralizing the resistant fraction and in demonstrating otherwise undetectable antiviral antibody.
如果将长期感染乳酸脱氢酶病毒（LDV）的小鼠的病毒血症血清先用乙醚或紫外线处理以灭活感染性病毒，就可以检测到中和抗体。然而，直到小鼠感染约2.5个月后才检测到大量抗体，并且抗体的存在并未导致慢性病毒血症的消除。从含有中和抗体的血清中分离出的病毒被发现对抗LDV中和作用具有相对抗性。进一步的研究表明，抗性病毒以感染性病毒 - 抗体复合物（致敏病毒）的形式存在。这种复合物的存在通过以下事实得以证明：在体内或体外暴露于小鼠抗LDV后持续存在的病毒部分很容易被山羊抗小鼠血清或山羊抗小鼠γ球蛋白中和，而先前未暴露于小鼠抗LDV的病毒对山羊抗小鼠血清的中和作用完全抗性。这些发现表明：（a）致敏可能在病毒对抗病毒抗体中和作用的抗性和敏感性中起重要作用，并且（b）抗γ球蛋白可能被证明在中和抗性部分和证明其他无法检测到的抗病毒抗体方面有用。
