己糖激酶和三羧酸循环中间产物对大鼠肝脏线粒体脂肪酸氧化及酮体生成的影响。
The effect of hexokinase and tricarboxylic acid-cycle intermediates on fatty acid oxidation and formation of ketone bodies by rat-liver mitochondria.
作者信息
Hird F J, Symons R H, Weidemann M J
出版信息
Biochem J. 1966 Feb;98(2):389-93. doi: 10.1042/bj0980389.
Abstract
- The oxidation of butyrate, hexanoate and octanoate by rat-liver mitochondria suspended in a tris-potassium chloride medium in the presence of malate and serum albumin has been investigated. 2. The oxidation of butyrate to acetoacetate was markedly decreased by the addition of a system competitive for ATP (hexokinase-glucose). 3. Serum albumin or tricarboxylic acid-cycle intermediates prevented the inhibition by hexokinase and in their presence a greater proportion of the oxygen consumption was contributed by the tricarboxylic acid cycle. The results suggest that the energy supply for fatty acid activation is either compartmentalized in a spatial or kinetic sense or there exists a special activating mechanism not involving ATP. 4. Malate and other tricarboxylic acid-cycle intermediates caused substantial reduction (to beta-hydroxybutyrate) of the acetoacetate formed during the oxidation of butyrate, hexanoate and octanoate.
摘要
- 研究了悬浮于含有苹果酸和血清白蛋白的三羟甲基氨基甲烷 - 氯化钾介质中的大鼠肝脏线粒体对丁酸、己酸和辛酸的氧化作用。2. 添加对ATP有竞争性的系统（己糖激酶 - 葡萄糖）后，丁酸氧化为乙酰乙酸的过程显著减少。3. 血清白蛋白或三羧酸循环中间产物可防止己糖激酶的抑制作用，在它们存在的情况下，三羧酸循环对氧消耗的贡献比例更大。结果表明，脂肪酸活化所需的能量供应在空间或动力学意义上是分隔的，或者存在一种不涉及ATP的特殊活化机制。4. 苹果酸和其他三羧酸循环中间产物使丁酸、己酸和辛酸氧化过程中形成的乙酰乙酸大量还原（生成β - 羟基丁酸）。
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The metabolism of short-chain fatty acids in the sheep. I. Fatty acid utilization and ketone body production by rumen epithelium and other tissues.绵羊体内短链脂肪酸的代谢。I. 瘤胃上皮及其他组织对脂肪酸的利用和酮体生成
Biochem J. 1952 May;51(2):251-8. doi: 10.1042/bj0510251.
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Activation of fatty acid oxidation by dihydrodiphosphopyridine nucleotide.二氢二磷酸吡啶核苷酸对脂肪酸氧化的激活作用。
J Biol Chem. 1951 May;190(1):361-8.
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The requirements for serum albumin metabolism in subcellular fractions of liver and brain.肝脏和脑亚细胞组分中血清白蛋白代谢的要求。
Biochim Biophys Acta. 1960 Jan 1;37:55-63. doi: 10.1016/0006-3002(60)90078-0.
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The metabolism of glucose and butyrate by the omasum of the sheep.绵羊皱胃对葡萄糖和丁酸的代谢
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J Biol Chem. 1964 Jun;239:1694-9.
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Carnitine in intermediary metabolism. The metabolism of fatty acid esters of carnitine by mitochondria.肉碱在中间代谢中的作用。线粒体对肉碱脂肪酸酯的代谢。
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